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. 1974 Apr;71(4):1128–1132. doi: 10.1073/pnas.71.4.1128

Synthesis of DNA in Human Lymphocytes: Possible Control Mechanism

Bertold R Fridlender 1, Estela Medrano 1, José Mordoh 1
PMCID: PMC388176  PMID: 4524623

Abstract

Partially purified, isolated nuclei from lymphocytes either stimulated or not stimulated by phytohemagglutinin can equally well synthesize DNA when [3H]dTTP is used as precursor. Studies of DNA polymerase activity in nuclei and cytoplasm from these cells showed that the enzyme can be detected in either stimulated or nonstimulated lymphocytes. In nonstimulated lymphocytes the uptake of thymidine is very low. The use of inhibitors such as cycloheximide, arbinosylcytosine, and actinomycin D showed that a parallel existed between thymidine uptake and DNA synthesis. All the conditions in which DNA synthesis was inhibited resulted also in an inhibition of thymidine uptake.

Keywords: phytohemagglutinin, isolated nuclei, DNA polymerase, thymidine transport, cycloheximide

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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