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. 1974 Apr;71(4):1174–1177. doi: 10.1073/pnas.71.4.1174

Purification and Properties of Two RNA Polymerases from Physarum polycephalum

Ann Baker Burgess 1,*, Richard R Burgess 1
PMCID: PMC388186  PMID: 4524629

Abstract

Two RNA polymerases have been purified from the slime mold Physarum polycephalum, one sensitive and one resistant to α-amanitin. Both enzymes are more active with denatured DNA than native DNA as a template and prefer Mn++ rather than Mg++ as a divalent cation. The α-amanitin-sensitive enzyme shows maximum activity at 0.15 M KCl, whereas the resistant enzyme is most active at very low ionic strength. Analysis of the resistant enzyme on polyacrylamide gels containing sodium dodecyl sulfate shows two subunits present in a 1:1 ratio with molecular weights of 205,000 and 125,000.

Keywords: slime mold, polypeptide subunits

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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