Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Apr;71(4):1202–1206. doi: 10.1073/pnas.71.4.1202

Relationship of a Proton Gradient to the Active Transport of Proline with Membrane Vesicles from Mycobacterium phlei

Thomas R Hinds 1, Arnold F Brodie 1
PMCID: PMC388192  PMID: 4364528

Abstract

Electron transport particles prepared from Mycobacterium phlei were depleted of bound coupling factors by washing with water in the absence of inorganic ions. The depleted electron transport particles were void of latent ATPase activity and were capable of oxidation, but were unable to support coupled phosphorylation. Nevertheless, the depleted electron transport particles were capable of substrate-induced active transport of proline. Changes in pH in response to substrate oxidation were measured in normal and depleted electron particles with bromthymol blue. A bromthymol blue response upon substrate oxidation was not observed with depleted electron transport particles. The level of oxidative phosphorylation with succinate or NADH oxidation was not reduced in the presence of proline, and proline did not have an effect upon the proton gradients formed by the oxidation of either succinate or NADH.

Keywords: electron transport, oxidative phosphorylation, coupling factor(s), latent ATPase

Full text

PDF
1202

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASANO A., BRODIE A. F. OXIDATIVE PHOSPHORYLATION IN FRACTIONATED BACTERIAL SYSTEMS. XIV. RESPIRATORY CHAINS OF MYCOBACTERIUM PHLEI. J Biol Chem. 1964 Dec;239:4280–4291. [PubMed] [Google Scholar]
  2. Asano A., Cohen N. S., Baker R. F., Brodie A. F. Orientation of the cell membrane in ghosts and electron transport particles of Mycobacterium phlei. J Biol Chem. 1973 May 25;248(10):3386–3397. [PubMed] [Google Scholar]
  3. BRODIE A. F. Oxidative phosphorylation in fractionated bacterial systems. I. Role of soluble factors. J Biol Chem. 1959 Feb;234(2):398–404. [PubMed] [Google Scholar]
  4. Barnes E. M., Jr, Kaback H. R. Beta-galactoside transport in bacterial membrane preparations: energy coupling via membrane-bounded D-lactic dehydrogenase. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1190–1198. doi: 10.1073/pnas.66.4.1190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chance B., Mela L. Energy-linked changes of hydrogen ion concentration in submitochondrial particles. J Biol Chem. 1967 Mar 10;242(5):830–844. [PubMed] [Google Scholar]
  6. Chance B., Mela L. Intramitochondrial pH changes in cation accumulation. Proc Natl Acad Sci U S A. 1966 May;55(5):1243–1251. doi: 10.1073/pnas.55.5.1243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harold F. M., Baarda J. R. Inhibition of membrane transport in Streptococcus faecalis by uncouplers of oxidative phosphorylation and its relationship to proton conduction. J Bacteriol. 1968 Dec;96(6):2025–2034. doi: 10.1128/jb.96.6.2025-2034.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harold F. M. Conservation and transformation of energy by bacterial membranes. Bacteriol Rev. 1972 Jun;36(2):172–230. doi: 10.1128/br.36.2.172-230.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Higashi T., Bogin E., Brodie A. F. Separation of a factor indispensable for coupled phosphorylation from the particulate fraction of Mycobacterium phlei. J Biol Chem. 1969 Jan 25;244(2):500–502. [PubMed] [Google Scholar]
  10. Hirata H., Asano A., Brodie A. F. Respiration dependent transport of proline by electron transport particles from mycobacterium phlei. Biochem Biophys Res Commun. 1971 Jul 16;44(2):368–374. doi: 10.1016/0006-291x(71)90609-7. [DOI] [PubMed] [Google Scholar]
  11. Kaback H. R., Barnes E. M., Jr Mechanisms of active transport in isolated membrane vesicles. II. The mechanism of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in membrane preparations from Escherichia coli. J Biol Chem. 1971 Sep 10;246(17):5523–5531. [PubMed] [Google Scholar]
  12. Kaback H. R., Milner L. S. Relationship of a membrane-bound D-(-)-lactic dehydrogenase to amino acid transport in isolated bacterial membrane preparations. Proc Natl Acad Sci U S A. 1970 Jul;66(3):1008–1015. doi: 10.1073/pnas.66.3.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kalra V. K., Murti C. R., Brodie A. F. Resolution and reconstitution of the succinoxidase pathway of Mycobacterium phlei. Arch Biochem Biophys. 1971 Dec;147(2):734–743. doi: 10.1016/0003-9861(71)90433-4. [DOI] [PubMed] [Google Scholar]
  14. Kashket E. R., Wilson T. H. Role of metabolic energy in the transport of -galactosides by Streptococcus lactis. J Bacteriol. 1972 Feb;109(2):784–789. doi: 10.1128/jb.109.2.784-789.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Klein W. L., Boyer P. D. Energization of active transport by Escherichia coli. J Biol Chem. 1972 Nov 25;247(22):7257–7265. [PubMed] [Google Scholar]
  16. Kosmakos F. C., Brodie A. F. pH profiles of proline transport with membrane vesicles from Mycobacterium phlei with artificial and natural electron donors. Biochem Biophys Res Commun. 1973 Apr 2;51(3):572–579. doi: 10.1016/0006-291x(73)91352-1. [DOI] [PubMed] [Google Scholar]
  17. Lombardi F. J., Kaback H. R. Mechanisms of active transport in isolated bacterial membrane vesicles. 8. The transport of amino acids by membranes prepared from Escherichia coli. J Biol Chem. 1972 Dec 25;247(24):7844–7857. [PubMed] [Google Scholar]
  18. Mitchell P., Moyle J., Smith L. Bromthymol blue as a pH indicator in mitochondrial suspensions. Eur J Biochem. 1968 Mar;4(1):9–19. doi: 10.1111/j.1432-1033.1968.tb00166.x. [DOI] [PubMed] [Google Scholar]
  19. Murthy P. S., Bogin E., Higashi T., Brodie A. F. Properties of the soluble malate-vitamin K reductase and associated phosphorylation. J Biol Chem. 1969 Jun 25;244(12):3117–3124. [PubMed] [Google Scholar]
  20. Pavlasova E., Harold F. M. Energy coupling in the transport of beta-galactosides by Escherichia coli: effect of proton conductors. J Bacteriol. 1969 Apr;98(1):198–204. doi: 10.1128/jb.98.1.198-204.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Simoni R. D., Shallenberger M. K. Coupling of energy to active transport of amino acids in Escherichia coli. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2663–2667. doi: 10.1073/pnas.69.9.2663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stock J., Roseman S. A sodium-dependent sugar co-transport system in bacteria. Biochem Biophys Res Commun. 1971 Jul 2;44(1):132–138. doi: 10.1016/s0006-291x(71)80168-7. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES