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. 1974 Apr;71(4):1244–1246. doi: 10.1073/pnas.71.4.1244

A Hyperglycemic Peptide from Pituitary Growth Hormone: Preparation with Pepsin and Assay in ob/ob Mice*

Ardis J Lostroh 1, M E Krahl 1
PMCID: PMC388201  PMID: 4598296

Abstract

A fragment that induces glucose intolerance in hereditarily obese mice has been prepared from pituitary growth hormone(sheep and human) by controlled digestion with pepsin in 0.05 M sodium acetate buffer, pH 3.7. The digest was purified by column chromatography on Bio-Gel P-6, followed by chromatography on Bio-Rex 70 and Bio-Gel P-2; in each successive step peptide was quantitated with the fluorescamine procedure. The fragment from sheep growth hormone has these characteristics: (1) It induces glucose intolerance in fasted ob/ob female mice when injected subcutaneously in a divided dose 15 min before, and concurrently with, glucose. (2) It is a basie peptide, as judged from its elution behavior on Bio-Rex 70, H+ form. (3) It does not crossreact with antiserum to human growth hormone in radioimmunoassay. Further purification and initial sequence determinations have been undertaken.

Keywords: fragments of growth hormone, glucose intolerance, peptic digest, fluorescamine, diabetogenic

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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