Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Apr;71(4):1267–1271. doi: 10.1073/pnas.71.4.1267

Viral DNA·RNA Hybrids in Cells Infected with Simian Virus: The Simian Virus 40 Transcriptional Intermediates

Marc Girard 1, Louise Marty 1, Simone Manteuil 1
PMCID: PMC388207  PMID: 4364531

Abstract

Brief labeling of cells infected with simian virus 40 with tritiated uridine resulted in the incorporation of part of the label into material that was soluble in 1 M NaCl and sensitive to KOH, with a buoyant density close to that of DNA. The properties of this material suggest that it represents single-stranded nascent molecules of messenger RNA of simian virus 40 hydrogen-bonded over a small portion of their length to viral DNA templates. The name “simian virus 40 transcriptional intermediate molecules” is suggested for these naturally occurring DNA·RNA hybrid molecules. The DNA in the hybrid seems to be in the form of replicative intermediate molecules.

Keywords: transcription, nascent messenger RNA

Full text

PDF
1267

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aloni Y., Attardi G. Expression of the mitochondrial genome in HeLa cells. XI. Isolation and characterization of transcription complexes of mitochondrial DNA. J Mol Biol. 1972 Sep 28;70(2):363–373. doi: 10.1016/0022-2836(72)90545-1. [DOI] [PubMed] [Google Scholar]
  2. Aloni Y. Extensive symmetrical transcription of Simian Virus 40 DNA in virus-yielding cells. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2404–2409. doi: 10.1073/pnas.69.9.2404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Aloni Y., Winocour E., Sachs L. Characterization of the simian virus 40-specific RNA in virus-yielding and transformed cells. J Mol Biol. 1968 Feb 14;31(3):415–429. doi: 10.1016/0022-2836(68)90418-x. [DOI] [PubMed] [Google Scholar]
  4. Carp R. I., Sauer G., Sokol F. The effect of actinomycin D on the transcription and replication of simian virus 40 deoxyribonucleic acid. Virology. 1969 Feb;37(2):214–226. doi: 10.1016/0042-6822(69)90201-3. [DOI] [PubMed] [Google Scholar]
  5. Cowan K., Tegtmeyer P., Anthony D. D. Relationship of replication and transcription of Simian Virus 40 DNA. Proc Natl Acad Sci U S A. 1973 Jul;70(7):1927–1930. doi: 10.1073/pnas.70.7.1927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fareed G. C., McKerlie M. L., Salzman N. P. Characterization of Simian virus 40 DNA component II during viral DNA replication. J Mol Biol. 1973 Feb 25;74(2):95–111. doi: 10.1016/0022-2836(73)90101-0. [DOI] [PubMed] [Google Scholar]
  7. Granboulan N., Girard M. Molecular weight of poliovirus ribonucleic acid. J Virol. 1969 Oct;4(4):475–479. doi: 10.1128/jvi.4.4.475-479.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hayashi M. N., Hayashi M. Participation of a DNA-RNA hybrid complex in in vivo genetic transcription. Proc Natl Acad Sci U S A. 1966 Mar;55(3):635–641. doi: 10.1073/pnas.55.3.635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hayashi M. A DNA-RNA complex as an intermediate of in vitro genetic transcription. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1736–1743. doi: 10.1073/pnas.54.6.1736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  11. Jaenisch R., Mayer A., Levine A. Replicating SV40 molecules containing closed circular template DNA strands. Nat New Biol. 1971 Sep 15;233(37):72–75. doi: 10.1038/newbio233072a0. [DOI] [PubMed] [Google Scholar]
  12. Khoury G., Byrne J. C., Martin M. A. Patterns of Simian Virus 40 DNA transcription after acute infection of permissive and nonpermissive cells. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1925–1928. doi: 10.1073/pnas.69.7.1925. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kit S., Dubbs D. R., Frearson P. M., Melnick J. L. Enzyme induction in SV40-infected green monkey kidney cultures. Virology. 1966 May;29(1):69–83. doi: 10.1016/0042-6822(66)90197-8. [DOI] [PubMed] [Google Scholar]
  14. Kourilsky P., Manteuil S., Zamansky M. H., Gros F. DNA-RNA hybridization at low temperature in the presence of urea. Biochem Biophys Res Commun. 1970 Nov 25;41(4):1080–1087. doi: 10.1016/0006-291x(70)90196-8. [DOI] [PubMed] [Google Scholar]
  15. Levine A. J., Kang H. S., Billheimer F. E. DNA replication in SV40 infected cells. I. Analysis of replicating SV40 DNA. J Mol Biol. 1970 Jun 14;50(2):549–568. doi: 10.1016/0022-2836(70)90211-1. [DOI] [PubMed] [Google Scholar]
  16. Lindstrom D. M., Dulbecco R. Strand orientation of simian virus 40 transcription in productively infected cells. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1517–1520. doi: 10.1073/pnas.69.6.1517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Magnusson G., Pigiet V., Winnacker E. L., Abrams R., Reichard P. RNA-linked short DNA fragments during polyoma replication. Proc Natl Acad Sci U S A. 1973 Feb;70(2):412–415. doi: 10.1073/pnas.70.2.412. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Manteuil S., Pages J., Stehelin D., Girard M. Replication of simian virus 40 deoxyribonucleic acid: analysis of the one-step growth cycle. J Virol. 1973 Jan;11(1):98–106. doi: 10.1128/jvi.11.1.98-106.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Oda K., Dulbecco R. Regulation of transcription of the SV40 DNA in productively infected and in transformed cells. Proc Natl Acad Sci U S A. 1968 Jun;60(2):525–532. doi: 10.1073/pnas.60.2.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pages J., Manteuil S., Stehelin D., Fiszman M., Marx M., Girard M. Relationship between replication of simian virus 40 DNA and specific events of the host cell cycle. J Virol. 1973 Jul;12(1):99–107. doi: 10.1128/jvi.12.1.99-107.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. RAPP F., BUTEL J. S., FELDMAN L. A., KITAHARA T., MELNICK J. L. DIFFERENTIAL EFFECTS OF INHIBITORS ON THE STEPS LEADING TO THE FORMATION OF SV40 TUMOR AND VIRUS ANTIGENS. J Exp Med. 1965 Jun 1;121:935–944. doi: 10.1084/jem.121.6.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rozenblatt S., Winocour E. Covalently linked cell and SV40-specific sequences in an RNA from productively infected cells. Virology. 1972 Nov;50(2):558–566. doi: 10.1016/0042-6822(72)90407-2. [DOI] [PubMed] [Google Scholar]
  23. Sambrook J., Sharp P. A., Keller W. Transcription of Simian virus 40. I. Separation of the strands of SV40 DNA and hybridization of the separated strands to RNA extracted from lytically infected and transformed cells. J Mol Biol. 1972 Sep 14;70(1):57–71. doi: 10.1016/0022-2836(72)90163-5. [DOI] [PubMed] [Google Scholar]
  24. Sambrook J., Shatkin A. J. Polynucleotide ligase activity in cells infected with simian virus 40, polyoma virus, or vaccinia virus. J Virol. 1969 Nov;4(5):719–726. doi: 10.1128/jvi.4.5.719-726.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sauer G. Apparent differences in transcriptional control in cells productively infected and transformed by SV40. Nat New Biol. 1971 Jun 2;231(22):135–138. doi: 10.1038/newbio231135a0. [DOI] [PubMed] [Google Scholar]
  26. Sebring E. D., Kelly T. J., Jr, Thoren M. M., Salzman N. P. Structure of replicating simian virus 40 deoxyribonucleic acid molecules. J Virol. 1971 Oct;8(4):478–490. doi: 10.1128/jvi.8.4.478-490.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sugino A., Okazaki R. RNA-linked DNA fragments in vitro. Proc Natl Acad Sci U S A. 1973 Jan;70(1):88–92. doi: 10.1073/pnas.70.1.88. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tegtmeyer P., Macasaet F. Simian virus 40 deoxyribonucleic acid synthesis: analysis by gel electrophoresis. J Virol. 1972 Oct;10(4):599–604. doi: 10.1128/jvi.10.4.599-604.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Tegtmeyer P. Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon. J Virol. 1972 Oct;10(4):591–598. doi: 10.1128/jvi.10.4.591-598.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Weinberg R. A., Warnaar S. O., Winocour E. Isolation and characterization of simian virus 40 ribonucleic acid. J Virol. 1972 Aug;10(2):193–201. doi: 10.1128/jvi.10.2.193-201.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Westphal H. SV40 DNA strand selection by Escherichia coli RNA polymerase. J Mol Biol. 1970 Jun 14;50(2):407–420. doi: 10.1016/0022-2836(70)90201-9. [DOI] [PubMed] [Google Scholar]
  32. Williams A. E., Vinograd J. The buoyant behavior of RNA and DNA in cesium sulfate solutions containing dimethylsulfoxide. Biochim Biophys Acta. 1971 Jan 28;228(2):423–439. doi: 10.1016/0005-2787(71)90048-7. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES