. Author manuscript; available in PMC: 2014 Nov 19.

Published in final edited form as: Biochemistry. 2013 Nov 4;52(46):10.1021/bi400816s. doi: 10.1021/bi400816s

Table 1.

SAXS-determined R_g and D_Max values for CPEB3 ribozyme with crowding and cosolute agents.


	R_g(Guinier) (Å)	R_g(GNOM) (Å)	D_Max (Å)	Excluded Volume (Å³)
	0.5 mM Mg²⁺

Buffer	23.6 + 0.1	23.6 + 0.4	80	44,555
20% PEG200	26.1 + 0.5	25.4 ±0.5	87	45,570
20% PEG8000	21.3 + 0.1	21.8 + 0.7	75	40,544

	10 mM Mg²⁺

Buffer	22.0 + 0.6	22.6 + 0.1	75	41,280
20% PEG8000	19.9 + 0.7	20.2 ± 0.8	67	30,877

Model of CPEB3 ribozyme gives R_g = 20.4 Å and D_max=73.8 Å. The 0.5 mM Mg²⁺ data are at 0.6 mg/mL RNA concentration, while the 10 mM Mg²⁺ data in 20% PEG8000 are at 0.2 mg/mL and the 10 mM Mg²⁺ data in buffer alone are at ~1 mg/mL immediately following size-exclusion chromatography. The SUPCOMB20 program was used to calculate the RMSD and average excluded volume.⁴⁰