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. 1974 Apr;71(4):1330–1333. doi: 10.1073/pnas.71.4.1330

Cholinergic Augmentation of Lymphocyte-Mediated Cytotoxicity. A Study of the Cholinergic Receptor of Cytotoxic T Lymphocytes

Terry B Strom *, Arthur J Sytkowski †, Charles B Carpenter *, John P Merrill *
PMCID: PMC388221  PMID: 4364534

Abstract

Cholinergic agonists have previously been shown to augment the ability of sensitized lymphocytes to injure cells bearing the sensitizing alleantigens. The cholinergic receptor of the attacking lymphocyte population has been studied with pharmacological manipulation of an in vitro system that quantitates the injury mediated by sensitized attacking cells upon target cells. The data reveal that muscarinic ligands are several orders of magnitude more potent than nicotinic agents in altering cytotoxicity.

Keywords: cGMP, muscarinic agents, nicotinic agents

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beránek R., Vyskocil F. The action of tubocurarine and atropine on the normal and denervated rat diaphragm. J Physiol. 1967 Jan;188(1):53–66. doi: 10.1113/jphysiol.1967.sp008123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bosmann H. B. Acetylcholine receptor. I. Identification and biochemical characteristics of a cholinergic receptor of guinea pig cerebral cortex. J Biol Chem. 1972 Jan 10;247(1):130–145. [PubMed] [Google Scholar]
  3. Brunner K. T., Mauel J., Cerottini J. C., Chapuis B. Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology. 1968 Feb;14(2):181–196. [PMC free article] [PubMed] [Google Scholar]
  4. Brunner K. T., Mauel J., Rudolf H., Chapuis B. Studies of allograft immunity in mice. I. Induction, development and in vitro assay of cellular immunity. Immunology. 1970 Apr;18(4):501–515. [PMC free article] [PubMed] [Google Scholar]
  5. CHANG C. C., LEE C. Y. ISOLATION OF NEUROTOXINS FROM THE VENOM OF BUNGARUS MULTICINCTUS AND THEIR MODES OF NEUROMUSCULAR BLOCKING ACTION. Arch Int Pharmacodyn Ther. 1963 Jul 1;144:241–257. [PubMed] [Google Scholar]
  6. Cerottini J. C., Brunner K. T. Reversible inhibition of lymphocyte-mediated cytotoxicity by cytochalasin B. Nat New Biol. 1972 Jun 28;237(78):272–273. doi: 10.1038/newbio237272a0. [DOI] [PubMed] [Google Scholar]
  7. Cerottini J. C., Nordin A. A., Brunner K. T. In vitro cytotoxic activity of thymus cells sensitized to alloantigens. Nature. 1970 Jul 4;227(5253):72–73. doi: 10.1038/227072a0. [DOI] [PubMed] [Google Scholar]
  8. Cerottini J. C., Nordin A. A., Brunner K. T. Specific in vitro cytotoxicity of thymus-derived lymphocytes sensitized to alloantigens. Nature. 1970 Dec 26;228(5278):1308–1309. doi: 10.1038/2281308a0. [DOI] [PubMed] [Google Scholar]
  9. Changeux J. P., Kasai M., Lee C. Y. Use of a snake venom toxin to characterize the cholinergic receptor protein. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1241–1247. doi: 10.1073/pnas.67.3.1241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. DAY M., VANE J. R. An analysis of the direct and indirect actions of drugs on the isolated guinea-pig ileum. Br J Pharmacol Chemother. 1963 Feb;20:150–170. doi: 10.1111/j.1476-5381.1963.tb01306.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ferrendelli J. A., Steiner A. L., McDougal D. B., Jr, Kipnis D. M. The effect of oxotremorine and atropine on cGMP and cAMP levels in mouse cerebral cortex and cerebellum. Biochem Biophys Res Commun. 1970 Nov 25;41(4):1061–1067. doi: 10.1016/0006-291x(70)90193-2. [DOI] [PubMed] [Google Scholar]
  12. Fischbach G. D., Cohen S. A. The distribution of acetylcholine sensitivity over uninnervated and innervated muscle fibers grown in cell culture. Dev Biol. 1973 Mar;31(1):147–162. doi: 10.1016/0012-1606(73)90326-6. [DOI] [PubMed] [Google Scholar]
  13. George W. J., Polson J. B., O'Toole A. G., Goldberg N. D. Elevation of guanosine 3',5'-cyclic phosphate in rat heart after perfusion with acetylcholine. Proc Natl Acad Sci U S A. 1970 Jun;66(2):398–403. doi: 10.1073/pnas.66.2.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Greene L. A., Sytkowski A. J., Vogel Z., Nirenberg M. W. -Bungarotoxin used as a probe for acetylcholine receptors of cultured neurones. Nature. 1973 May 18;243(5403):163–166. doi: 10.1038/243163a0. [DOI] [PubMed] [Google Scholar]
  15. Henney C. S., Bourne H. R., Lichtenstein L. M. The role of cyclic 3',5' adenosine monophosphate in the specific cytolytic activity of lymphocytes. J Immunol. 1972 Jun;108(6):1526–1534. [PubMed] [Google Scholar]
  16. Henney C. S., Lichtenstein L. M. The role of cyclic AMP in the cytolytic activity of lymphocytes. J Immunol. 1971 Aug;107(2):610–612. [PubMed] [Google Scholar]
  17. Illiano G., Tell G. P., Siegel M. E., Cuatrecasas P. Guanosine 3':5'-cyclic monophosphate and the action of insulin and acetylcholine. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2443–2447. doi: 10.1073/pnas.70.8.2443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kuo J. F., Lee T. P., Reyes P. L., Walton K. G., Donnelly T. E., Jr, Greengard P. Cyclic nucleotide-dependent protein kinases. X. An assay method for the measurement of quanosine 3',5'-monophosphate in various biological materials and a study of agents regulating its levels in heart and brain. J Biol Chem. 1972 Jan 10;247(1):16–22. [PubMed] [Google Scholar]
  19. Mauel J., Rudolf H., Chapuis B., Brunner K. T. Studies of allograft immunity in mice. II. Mechanism of target cell inactivation in vitro by sensitized lymphocytes. Immunology. 1970 Apr;18(4):517–535. [PMC free article] [PubMed] [Google Scholar]
  20. Plaut M., Lichtenstein L. M., Henney C. S. Studies on the mechanism of lymphocyte-mediated cytolysis. 3. The role of microfilaments and microtubules. J Immunol. 1973 Mar;110(3):771–780. [PubMed] [Google Scholar]
  21. Strom T. B., Carpenter C. B., Garovoy M. R., Austen K. F., Merrill J. P., Kaliner M. The modulating influence of cyclic nucleotides upon lymphocyte-mediated cytotoxicity. J Exp Med. 1973 Aug 1;138(2):381–393. doi: 10.1084/jem.138.2.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Strom T. B., Deisseroth A., Morganroth J., Carpenter C. B., Merrill J. P. Alteration of the cytotoxic action of sensitized lymphocytes by cholinergic agents and activators of adenylate cyclase. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2995–2999. doi: 10.1073/pnas.69.10.2995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Strom T. B., Garovoy M. R., Carpenter D. B., Merrill J. P. Microtubule function in immune and nonimmune lymphocyte-mediated cytotoxicity. Science. 1973 Jul 13;181(4095):171–173. doi: 10.1126/science.181.4095.171. [DOI] [PubMed] [Google Scholar]
  24. Vogel Z., Sytkowski A. J., Nirenberg M. W. Acetylcholine receptors of muscle grown in vitro. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3180–3184. doi: 10.1073/pnas.69.11.3180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yamashita K., Field J. B. Elevation of cyclic guanosine 3',5'-monophosphate levels in dog thyroid slices caused by acetylcholine and sodium fluoride. J Biol Chem. 1972 Nov 10;247(21):7062–7066. [PubMed] [Google Scholar]

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