Abstract
Male strains of the water mold Achlya ambisexualis produce antheridial hyphae in response to the steroid hormone antheridiol. The antheridial hypha is postulated to be initiated through a localized wall softening with the enzyme cellulase. Freeze-etch studies of hormone-treated hyphae were conducted to determine if aggregates of vesicles are induced at the location of new antheridial hyphae. Localized aggregates of vesicles were found in conjunction with areas of wall thinning. These data suggest that the processes of vesiculation and secretion provide a mechanism for concentration of cellulase at the site of initiation of antheridial hyphae.
Keywords: water molds, steroid hormones, vesicles, freeze-etching
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Edwards J. A., Mills J. S., Sundeen J., Fried J. H. The synthesis of the fungal sex hormone antheridiol. J Am Chem Soc. 1969 Feb 26;91(5):1248–1249. doi: 10.1021/ja01033a055. [DOI] [PubMed] [Google Scholar]
- Kane B. E., Jr, Reiskind J. B., Mullins J. T. Hormonal control of sexual morphogenesis in achlya: dependence on protein and ribonucleic Acid syntheses. Science. 1973 Jun 15;180(4091):1192–1193. doi: 10.1126/science.180.4091.1192. [DOI] [PubMed] [Google Scholar]
- Matile P., Cortat M., Wiemken A., Frey-Wyssling A. Isolation of glucanase-containing particles from budding Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1971 Mar;68(3):636–640. doi: 10.1073/pnas.68.3.636. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McMorris T. C., Barksdale A. W. Isolation of a sex hormone from the water mould Achlya bisexualis. Nature. 1967 Jul 15;215(5098):320–321. doi: 10.1038/215320a0. [DOI] [PubMed] [Google Scholar]
- Moor H. Endoplasmic reticulum as the initiator of bud formation in yeast (S. cerevisiae). Arch Mikrobiol. 1967 Jun 6;57(2):135–146. doi: 10.1007/BF00408697. [DOI] [PubMed] [Google Scholar]
- Mullins J. T. Lateral branch formation and cellulase production in the water molds. Mycologia. 1973 Sep-Oct;65(5):1007–1014. [PubMed] [Google Scholar]
- Sentandreu R., Northcote D. H. The formation of buds in yeast. J Gen Microbiol. 1969 Mar;55(3):393–398. doi: 10.1099/00221287-55-3-393. [DOI] [PubMed] [Google Scholar]
- Thomas D des S., Mullins J. T. Role of enzymatic wall-softening in plant morphogenesis: hormonal induction in Achlya. Science. 1967 Apr 7;156(3771):84–85. doi: 10.1126/science.156.3771.84. [DOI] [PubMed] [Google Scholar]