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. 1974 Apr;71(4):1446–1450. doi: 10.1073/pnas.71.4.1446

In Vitro Synthesis of Mouse Neuroblastoma Tubulin

Gerhard Wiche 1,2,3, Claire Zomzely-Neurath 1,2,3, Arthur J Blume 1,2,3,*
PMCID: PMC388246  PMID: 4524647

Abstract

Polyribosomes were isolated from a clonal line of mouse neuroblastoma grown in culture. In a heterologous in vitro system containing rat brain components, these polyribosomes were shown to direct the synthesis of neuroblastoma tubulin. Identification of the tubulin synthesized in vitro was achieved by coelectrophoresis with native neuroblastoma tubulin on sodium dodecyl sulfate polyacrylamide gels, immunoprecipitation, and demonstration of specific aggregation. Tubulin accounted for 2% of the total proteins synthesized. This in vitro protein synthesizing system offers a model for studying possible translational control mechanisms regulating the synthesis of proteins involved in nerve cell function.

Keywords: tissue culture, polyribosomes, gel electrophoresis

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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