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. 1974 Apr;71(4):1489–1492. doi: 10.1073/pnas.71.4.1489

Enhancement by Carbachol of Transmitter Release from Motor Nerve Terminals

Michael D Miyamoto 1, Robert L Volle 1
PMCID: PMC388255  PMID: 4364541

Abstract

In the endplates of rat phrenic nerve-diaphragm, application of the acetylcholine-like compound, carbachol, causes a marked increase in transmitter release, as measured electrophysiologically using miniature endplate potential frequency. Washing out of carbachol reverses the increase in frequency. The ability of carbachol to increase transmitter release is greatly enhanced by perfusion of the preparation with Ringer solution containing elevated K+. At concentrations of carbachol greater than 30 μM, the onset of the postjunctional blocking action of carbachol is too rapid and obscures the increase in miniature potential frequency. The rate of increase in transmitter release is dependent on the concentration of carbachol applied and can be antagonized by d-tubocurarine (10-60 nM) and other blocking compounds. These findings, in contrast to previous reports, indicate that cholinergic nerve endings, like adrenergic nerve endings, respond to applied acetylcholine-like drugs with measurable increases in transmitter output.

Keywords: miniature endplate potential frequency, cholinomimetics, nerve terminal depolarization, prejunctional cholinoceptive sites, cholinergic neurosecretion

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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