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. 1974 Apr;71(4):1554–1558. doi: 10.1073/pnas.71.4.1554

Synthesis of Nerve Growth Factor by L and 3T3 Cells in Culture

Joel Oger 1,2,3,4, Barry G W Arnason 1,2,3,4,*, Nicholas Pantazis 1,2,3,4, James Lehrich 1,2,3,4, Michael Young 1,2,3,4,*
PMCID: PMC388269  PMID: 4524658

Abstract

Mouse submaxillary gland nerve growth factor (NGF) has been covalently joined to bacteriophage and the resulting phage conjugates remain biologically active in stimulating neurite extension from sensory ganglia. A sensitive bacteriophage immunoassay has been developed to measure concentrations of NGF as low as 1 ng/ml. With this method, we find that mouse L and 3T3 cells in culture produce a biologically active nerve growth factor that is immunologically similar if not identical to mouse submaxillary gland NGF. Since L cells are known to be a source of “conditioned medium” for tissue culture, it could be that one or more of the conditioning factor activities secreted by these cells are due to NGF itself.

Keywords: conditioned culture medium, bacteriophage immunoassay, fibroblasts

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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