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. 1974 Apr;71(4):1559–1563. doi: 10.1073/pnas.71.4.1559

A Functional Mitotic Spindle Prepared from Mammalian Cells in Culture

W Zacheus Cande *, Judith Snyder *, Diana Smith *, Keith Summers †, J R McIntosh *
PMCID: PMC388270  PMID: 4524659

Abstract

Mitotic cells lysed into solutions of polymerizable microtubule protein contain a spindle which is similar to the living spindle in two respects: it will lose and gain birefringence when cooled and warmed, and it will move anaphase chromosomes to the opposite ends of the cell. Early anaphase cells lysed into buffers containing high molecular weight polyethylene glycol and nucleotide triphosphates will continue chromosome motion and spindle elongation in the absence of exogenous spindle subunits. These results suggest that while spindle growth requires microtubule polymerization, anaphase motions do not.

Keywords: microtubule assembly, motility, anaphase in vitro

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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