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. 1974 May;71(5):1748–1752. doi: 10.1073/pnas.71.5.1748

Non-Selective Inhibition of Transformed Cell Growth by a Protease Inhibitor

Iih-Nan Chou 1,2,3,4, Paul H Black 1,2,3,4, Richard O Roblin 1,2,3,4
PMCID: PMC388316  PMID: 4365571

Abstract

The protease inhibitors N-tosyl-L-phenylalanylchloromethyl ketone (TPCK) and N-tosyl-L-lysylchloromethyl ketone (TLCK) have previously been shown to selectively inhibit growth of simian virus 40-transformed cells, suggesting that proteolytic enzymes play a role in loss of cellular growth control following viral transformation. In contrast, this study shows that TPCK-mediated growth inhibition is non-selective, since the growth of both simian virus 40-transformed and untransformed 3T3 cells is similarly reduced by TPCK treatment. Under certain conditions, TPCK treatment of simian virus 40-transformed cells yields a reversible “growth plateau” condition which mimics, but is not equivalent to, contact inhibition of growth. The growth inhibitory effects of TPCK are due to inhibition of protein synthesis, since TPCK treatment resulted in a diminution of protein synthesis and since the “growth plateau” effect was also observed in cultures treated with cycloheximide.

Keywords: contact inhibition, 3T3 cells, simian virus 40 transformation, N-tosyl-L-phenylalanylchloromethyl ketone

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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