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. 1974 May;71(5):1785–1789. doi: 10.1073/pnas.71.5.1785

Distribution of Repetitive and Nonrepetitive Sequence Transcripts in HeLa mRNA

William H Klein *,‡, William Murphy *,‡, Giuseppe Attardi *,‡, Roy J Britten *, Eric H Davidson *,‡,†
PMCID: PMC388325  PMID: 4525461

Abstract

Polyadenylated messenger RNA extracted from HeLa cells was hybridized with a mass excess of HeLa DNA. The kinetics of the hybridization reaction demonstrated that most of the messenger RNA is transcribed from nonrepetitive DNA. The amount of messenger RNA hybridized to DNA was measured both with and without prior RNase treatment. Comparison of the results indicates that within the limits of detection, HeLa messenger RNA does not contain repetitive sequence elements covalently linked to nonrepetitive sequence transcripts. However, a small fraction of the HeLa messenger RNA preparation is transcribed entirely from repetitive DNA sequences. This fraction represents about 6% of the total polyadenylated messenger RNA preparation.

Keywords: polyadenylated mRNA, hydroxyapatite, RNA·DNA hybridization

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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