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. 1974 May;71(5):1945–1948. doi: 10.1073/pnas.71.5.1945

Proton Nuclear Magnetic Resonance Investigations and Ring Current Calculations of Guanine N-1 and Thymine N-3 Hydrogen-Bonded Protons in Double-Helical Deoxyribonucleotides in Aqueous Solution

Dinshaw J Patel 1, Alan E Tonelli 1
PMCID: PMC388359  PMID: 4525304

Abstract

Methods are outlined for assigning the guanine-N1H and thymine-N3H protons to particular base pairs in the proton nuclear magnetic resonance spectra of double-stranded oligodeoxyribonucleotides of known sequence in aqueous solution. Ring current calculations have been used to evaluate the upfield shifts of the guanine-N1H and thymine-N3H protons from the pyrimidine and purine rings of nearest-neighbor base pairs in DNA B-type double-helical structures. Chemical shifts of 13.6 ± 0.1 ppm and 14.6 ± 0.2 ppm are assigned to the guanine-N1H proton of an isolated G·C base pair and the thymine-N3H proton of an isolated A·T base pair, respectively.

Keywords: base pairs, actinomycin D

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1945

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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