Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 May;71(5):1987–1991. doi: 10.1073/pnas.71.5.1987

Mechanism of Enzymic Acetylation of des-Acetyl Citrate Lyase

H Schmellenkamp 1, H Eggerer 1
PMCID: PMC388369  PMID: 4365579

Abstract

A new enzyme, acetate:SH-[acyl-carrier-protein] enzyme ligase (AMP), has been purified about 30-fold from cell-free extracts of Klebsiella aerogenes. The enzyme, in the presence of acetate and ATP, catalyzes the acetylation of enzymically inactive des-acetyl citrate lyase to enzymically active citrate lyase (EC 4.1.3.6). Acetate and ATP could be replaced by acetyl adenylate. Acetyl-CoA can act as acetyl donor in this activation only if trace amounts of adenine nucleotides and auxiliary enzymes are present. These allow formation of acetyl adenylate in acetate- and ATP-generating reactions.

Keywords: enzymic activation, acetyl adenylate, acetate:enzyme ligase

Full text

PDF
1987

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEINERT H., GREEN D. E., HELE P., HIFT H., VON KORFF R. W., RAMAKRISHNAN C. V. The acetate activating enzyme system of heart muscle. J Biol Chem. 1953 Jul;203(1):35–45. [PubMed] [Google Scholar]
  2. BERG P. Acyl adenylates; an enzymatic mechanism of acetate activation. J Biol Chem. 1956 Oct;222(2):991–1013. [PubMed] [Google Scholar]
  3. Bowen T. J., Mortimer M. G. Sub-units of citrate oxaloacetate-lyase. Eur J Biochem. 1971 Nov 11;23(2):262–266. doi: 10.1111/j.1432-1033.1971.tb01617.x. [DOI] [PubMed] [Google Scholar]
  4. Buckel W., Buschmeier V., Eggerer H. Der Wirkungsmechanismus der Citrat-Lyase aus Klebsiella aerogenes. Hoppe Seylers Z Physiol Chem. 1971 Sep;352(9):1195–1205. [PubMed] [Google Scholar]
  5. Buckel W., Eggerer H. Zur optischen Bestimmung von Citrat-Synthase und von Acetyl-Coenzym A. Biochem Z. 1965 Nov 5;343(1):29–43. [PubMed] [Google Scholar]
  6. Buckel W., Ziegert K., Eggerer H. Acetyl-CoA-dependent cleavage of citrate on inactivated citrate lyase. Eur J Biochem. 1973 Aug 17;37(2):295–304. doi: 10.1111/j.1432-1033.1973.tb02988.x. [DOI] [PubMed] [Google Scholar]
  7. Dimroth P., Dittmar W., Walther G., Eggerer H. The acyl-carrier protein of citrate lyase. Eur J Biochem. 1973 Aug 17;37(2):305–315. doi: 10.1111/j.1432-1033.1973.tb02989.x. [DOI] [PubMed] [Google Scholar]
  8. JONES M. E., BLACK S., FLYNN R. M., LIPMANN F. Acetyl coenzyme a synthesis through pyrophosphoryl split of adenosine triphosphate. Biochim Biophys Acta. 1953 Sep-Oct;12(1-2):141–149. doi: 10.1016/0006-3002(53)90133-4. [DOI] [PubMed] [Google Scholar]
  9. Schmellenkamp H., Eggerer H. Enzymkatalysierte Reaktivierung inaktiver Citrat-Lyase. Hoppe Seylers Z Physiol Chem. 1972 Oct;353(10):1563–1564. [PubMed] [Google Scholar]
  10. Singh M., Böttger B., Stewart C., Brooks G. C., Srere P. A. S-acetyl phosphopantetheine: deacetyl citrate lyase S-acetyl transferase from Klebsiella aerogenes. Biochem Biophys Res Commun. 1973 Jul 2;53(1):1–9. doi: 10.1016/0006-291x(73)91392-2. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES