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. 1974 May;71(5):1995–1998. doi: 10.1073/pnas.71.5.1995

Amino-Acid Sequence of an Allotype b4 Light Chain from a Rabbit Antibody to Streptococcal Carbohydrate

K C S Chen 1, T J Kindt 1, R M Krause 1
PMCID: PMC388371  PMID: 4525311

Abstract

The complete amino-acid sequence was determined for a rabbit-antibody light chain of allotype b4. The light chain was recovered from approximately 20 g of homogeneous antibody to group C streptococcal carbohydrate, which was obtained from an immunized rabbit. Several enzymic digestions were used to obtain the overlapping peptides necessary for the sequence determination. The L chain has 210 amino-acid residues, as determined by sequence analysis, and a molecular mass of 23,060 daltons. This antibody light chain contains seven half-cystine residues characteristic of the κB light-chain class. The amino-terminal sequence was determined to be Ala-Asp-Ile-Val-Met, indicating that the chain belongs to the VκI subgroup.

Keywords: protein structure, homogeneous antibody

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1995

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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