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. 1974 May;71(5):1999–2002. doi: 10.1073/pnas.71.5.1999

Reconstruction of Mammalian Cells from Nuclear and Cytoplasmic Components Separated by Treatment with Cytochalasin B

George Veomett 1, D M Prescott 1, Jerry Shay 1, K R Porter 1
PMCID: PMC388372  PMID: 4525471

Abstract

Mouse L929 cells were separated into enucleated cytoplasmic components (cytoplasts) and nucleated subcellular fractions (karyoplasts) in the presence of cytochalasin B. Karyoplasts from cells containing tritiated nuclei were fused, using inactivated Sendai virus, to cytoplasts from cells containing large (1.0-μm diameter) latex spheres in the cytoplasm. Mononucleated cells containing radioactive nuclei and large latex spheres in the cytoplasm were observed among the products of the fusion reaction. Some of these cells were in mitotic configurations. The results indicate that cells capable of undergoing mitosis can be reconstructed from the products of cellular enucleation in the presence of cytochalasin B.

Keywords: latex spheres, cytoplasmic labels, Sendai virus

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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