Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 May;71(5):2003–2007. doi: 10.1073/pnas.71.5.2003

An RNA-Dependent Nucleoside Triphosphate Phosphohydrolase (ATPase) Associated with Rho Termination Factor

Carolyn Lowery-Goldhammer 1, John P Richardson 1
PMCID: PMC388373  PMID: 4365581

Abstract

Highly purified rho termination factor from Escherichia coli catalyzes an RNA-dependent hydrolysis of ribonucleoside triphosphates to nucleoside diphosphates and inorganic phosphate. In the presence of poly(C), a specific activity of 100 μmole of ATP hydrolyzed per min/mg has been measured. The phosphohydrolase activity appears to be associated with the protein responsible for termination of RNA synthesis, but a functional relationship between the two activities is not yet evident. Hydrolysis of nucleoside triphosphates occurs in the absence of termination and without any extensive degradation of RNA.

Keywords: Escherichia coli, RNA polymerase, transcription

Full text

PDF
2003

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beckmann J. S., Tichauer Y., Daniel V., Littauer U. Z. Binding of the termination factor rho to DNA. Biochem Biophys Res Commun. 1971 May 21;43(4):806–813. doi: 10.1016/0006-291x(71)90688-7. [DOI] [PubMed] [Google Scholar]
  2. Clausen T. Measurement of 32P activity in a liquid scintillation counter without the use of scintillator. Anal Biochem. 1968 Jan;22(1):70–73. doi: 10.1016/0003-2697(68)90260-1. [DOI] [PubMed] [Google Scholar]
  3. Darlix J. L., Sentenac A., Fromageot P. Binding of termination factor RHO to RNA polymerase and DNA. FEBS Lett. 1971 Mar 5;13(3):165–168. doi: 10.1016/0014-5793(71)80226-0. [DOI] [PubMed] [Google Scholar]
  4. De Crombrugghe B., Adhya S., Gottesman M., Pastan I. Effect of Rho on transcription of bacterial operons. Nat New Biol. 1973 Feb 28;241(113):260–264. doi: 10.1038/newbio241260a0. [DOI] [PubMed] [Google Scholar]
  5. Ebisuzaki K., Behme M. T., Senior C., Shannon D., Dunn D. An alternative approach to the study of new enzymatic reactions involving DNA (DNA-dependent ATPases-purification-properties-E. coli). Proc Natl Acad Sci U S A. 1972 Feb;69(2):515–519. doi: 10.1073/pnas.69.2.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eskin B., Linn S. The deoxyribonucleic acid modification and restriction enzymes of Escherichia coli B. J Biol Chem. 1972 Oct 10;247(19):6192–6196. [PubMed] [Google Scholar]
  7. Glynn I. M., Chappell J. B. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity. Biochem J. 1964 Jan;90(1):147–149. doi: 10.1042/bj0900147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goldberg A. R., Hurwitz J. Studies on termination of in vitro ribonucleic acid synthesis by rho factor. J Biol Chem. 1972 Sep 10;247(17):5637–5645. [PubMed] [Google Scholar]
  9. Goldmark P. J., Linn S. Purification and properties of the recBC DNase of Escherichia coli K-12. J Biol Chem. 1972 Mar 25;247(6):1849–1860. [PubMed] [Google Scholar]
  10. Horne J. R., Erdmann V. A. ATPase and GTPase activities associated with a specific 5S RNA-protein complex. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2870–2873. doi: 10.1073/pnas.70.10.2870. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Losick R. In vitro transcription. Annu Rev Biochem. 1972;41:409–446. doi: 10.1146/annurev.bi.41.070172.002205. [DOI] [PubMed] [Google Scholar]
  12. Minkley E. G., Jr Letter: functional form of RNA synthesis termination factor rho. J Mol Biol. 1973 Aug 15;78(3):577–580. doi: 10.1016/0022-2836(73)90479-8. [DOI] [PubMed] [Google Scholar]
  13. Oda T., Takanami M. Observations on the structure of the termination factor rho and its attachment to DNA. J Mol Biol. 1972 Nov 28;71(3):799–802. doi: 10.1016/s0022-2836(72)80041-x. [DOI] [PubMed] [Google Scholar]
  14. PULLMAN M. E., PENEFSKY H. S., DATTA A., RACKER E. Partial resolution of the enzymes catalyzing oxidative phosphorylation. I. Purification and properties of soluble dinitrophenol-stimulated adenosine triphosphatase. J Biol Chem. 1960 Nov;235:3322–3329. [PubMed] [Google Scholar]
  15. Roberts J. W. Termination factor for RNA synthesis. Nature. 1969 Dec 20;224(5225):1168–1174. doi: 10.1038/2241168a0. [DOI] [PubMed] [Google Scholar]
  16. Takanami M. Analysis of the 5'-terminal nucleotide sequences of ribonucleic acids 1. the 5'-termini of Excherichia coli ribosomal RNA. J Mol Biol. 1967 Jan 28;23(2):135–148. doi: 10.1016/s0022-2836(67)80022-6. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES