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. 1974 May;71(5):2022–2026. doi: 10.1073/pnas.71.5.2022

The Enzymatic Release of O6-methylguanine and 3-methyladenine from DNA Reacted with the Carcinogen N-methyl-N-nitrosourea

D M Kirtikar 1, D A Goldthwait 1
PMCID: PMC388377  PMID: 4600266

Abstract

Endonuclease II (deoxyribonucleate oligonucleotidohydrolase, EC 3.1.4.30) of Escherichia coli has been shown to break phosphodiester bonds in alkylated DNA and depurinated DNA. The hypothesis that depurination is a step in the mechanism of the reaction with alkylated DNA is supported by in vitro experiments with DNA reacted with N-methyl-N-nitrosourea. Endonuclease II releases O6-methylguanine and 3-methyladenine, but not 7-methylguanine, from DNA that has been methylated by the carcinogen N-methyl-N-nitrosourea.

Keywords: DNA repair, methylnitrosourea, endonuclease II, chemical carcinogenesis

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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