Abstract
When Ehrlich ascites tumor cells fused with UV-inactivated HVJ (Sendai virus) were cultured, the viral envelope antigens, which had been integrated at random into the cell membranes, accumulated at one site on the fused cell. The site showed a specific structure, namely, a projection of the cytoplasm from the spherical fused cell surface with clusters of many long microvillilike protrusions from it. When the fused cells were conjugated with antibody against Ehrlich ascites tumor cells and cultured, the antigen-antibody complex also accumulated on the projection.
Associated with the development of the projection and accumulation of viral antigens, vacuoles developed at the base of the projection in the cytoplasm of the fused cells in culture. The development of the projection and vacuoles and the accumulation of viral antigens were all inhibited by glucose, mannose, galactose, sodium azide, and cycloheximide, and these inhibitions were reversible. All these structures were observed in multinucleated cells, but few in mononucleated cells after reaction with HVJ (Sendai virus). We consider that these three phenomena correlate with each other and may result from changes in cell-membrane systems caused by fusion of cells.
Keywords: cap formation, projection of cytoplasm, microvilli, vacuoles
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- Frye L. D., Edidin M. The rapid intermixing of cell surface antigens after formation of mouse-human heterokaryons. J Cell Sci. 1970 Sep;7(2):319–335. doi: 10.1242/jcs.7.2.319. [DOI] [PubMed] [Google Scholar]
- Loor F., Forni L., Pernis B. The dynamic state of the lymphocyte membrane. Factors affecting the distribution and turnover of surface immunoglobulins. Eur J Immunol. 1972 Jun;2(3):203–212. doi: 10.1002/eji.1830020304. [DOI] [PubMed] [Google Scholar]
- Morgan C., Howe C. Structure and development of viruses as observed in the electron microscope. IX. Entry of parainfluenza I (Sendai) virus. J Virol. 1968 Oct;2(10):1122–1132. doi: 10.1128/jvi.2.10.1122-1132.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OKADA Y. Analysis of giant polynuclear cell formation caused by HVJ virus from Ehrlich's ascites tumor cells. I. Microscopic observation of giant polynuclear cell formation. Exp Cell Res. 1962 Feb;26:98–107. doi: 10.1016/0014-4827(62)90205-7. [DOI] [PubMed] [Google Scholar]
- OKADA Y., TADOKORO J. Analysis of giant polynuclear cell formation caused by HVJ virus from Ehrlich's ascites tumor cells. II. Quantitative analysis of giant polynuclear cell formation. Exp Cell Res. 1962 Feb;26:108–118. doi: 10.1016/0014-4827(62)90206-9. [DOI] [PubMed] [Google Scholar]
- Okada Y. Factors in fusion of cells by HVJ. Curr Top Microbiol Immunol. 1969;48:102–128. doi: 10.1007/978-3-642-46163-7_5. [DOI] [PubMed] [Google Scholar]
- Okada Y., Murayama F. Fusion of cells by HVJ: requirement of concentration of virus particles at the site of contact of two cells for fusion. Exp Cell Res. 1968 Sep;52(1):34–42. doi: 10.1016/0014-4827(68)90545-4. [DOI] [PubMed] [Google Scholar]
- Okada Y., Murayama F. Requirement of calcium ions for the cell fusion reaction of animal cells by HVJ. Exp Cell Res. 1966 Nov-Dec;44(2):527–551. doi: 10.1016/0014-4827(66)90458-7. [DOI] [PubMed] [Google Scholar]
- Yamanaka T., Okada Y. Cultivation of fused cells resulting from treatment of cells with HVJ. II. Division of binucleated cells resulting from fusion of two KB cells by HVJ. Exp Cell Res. 1968 Mar;49(3):461–469. doi: 10.1016/0014-4827(68)90195-x. [DOI] [PubMed] [Google Scholar]