Abstract
An ascites form of malignant transformed lymphoma cells were treated in vitro with liposomes of 1:1 lecithin-cholesterol in order to increase the cholesterol level of the cell-surface membranes and thereby to increase the rigidity of the lipid layer. This treatment was found to inhibit the rate of killing by ascites tumor after intraperitoneal inoculation into adult mice of 104 and 105 treated cells per animal. With 103 treated cells per animal, full survival was observed up to 90 days after inoculation, whereas with the same number of untreated cells all infected mice died within 30 days after inoculation. An analogous treatment of the malignant lymphoma cells with liposomes of pure lecithin did not result in any appreciable inhibitory effect on the ascites tumor development in vivo, as initiated by inoculation of 105, 104, or 103 cells per animal.
Keywords: malignant transformation, membrane fluidity, lipid layers, leukemia
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bretscher M. S. Membrane structure: some general principles. Science. 1973 Aug 17;181(4100):622–629. doi: 10.1126/science.181.4100.622. [DOI] [PubMed] [Google Scholar]
- Bruckdorfer K. R., Demel R. A., De Gier J., van Deenen L. L. The effect of partial replacements of membrane cholesterol by other steroids on the osmotic fragility and glycerol permeability of erythrocytes. Biochim Biophys Acta. 1969 Jul 15;183(2):334–345. doi: 10.1016/0005-2736(69)90089-3. [DOI] [PubMed] [Google Scholar]
- Bruckdorfer K. R., Edwards P. A., Green C. Properties of aqueous dispersions of phospholipid and cholesterol. Eur J Biochem. 1968 May;4(4):506–511. doi: 10.1111/j.1432-1033.1968.tb00241.x. [DOI] [PubMed] [Google Scholar]
- Bruckdorfer K. R., Graham J. M., Green C. The incorporation of steroid molecules into lecithin sols, beta-lipoproteins and cellular membranes. Eur J Biochem. 1968 May;4(4):512–518. doi: 10.1111/j.1432-1033.1968.tb00242.x. [DOI] [PubMed] [Google Scholar]
- Gitler C. Plasticity of biological membranes. Annu Rev Biophys Bioeng. 1972;1:51–92. doi: 10.1146/annurev.bb.01.060172.000411. [DOI] [PubMed] [Google Scholar]
- Inbar M., Ben-Bassat H., Sachs L. Inhibition of ascites tumor development by concanavalin A. Int J Cancer. 1972 Jan 15;9(1):143–149. doi: 10.1002/ijc.2910090117. [DOI] [PubMed] [Google Scholar]
- KLEIN E., KLEIN G. ANTIGENIC PROPERTIES OF LYMPHOMAS INDUCED BY THE MOLONEY AGENT. J Natl Cancer Inst. 1964 Mar;32:547–568. [PubMed] [Google Scholar]
- PARSONS J., WYCOFF H. D. Chromatographic microassay for cholesterol and cholesterol esters. Science. 1957 Feb 22;125(3243):347–348. doi: 10.1126/science.125.3243.347. [DOI] [PubMed] [Google Scholar]
- Teichberg V. I., Shinitzky M. Fluorescence polarization studies of lysozyme and lysozyme-saccharide complexes. J Mol Biol. 1973 Mar 15;74(4):519–531. doi: 10.1016/0022-2836(73)90044-2. [DOI] [PubMed] [Google Scholar]