Abstract
Using a method of gradient gel electrophoresis coupled with autoradiography, we have analyzed the polypeptide composition of the proteins being transported down the axons of the projecting neurons of the rabbit retina. This analysis reveals: (1) the molecular weight distribution of 43 transported polypeptides; (2) the existence of at least four components of intra-axonal protein transport, each characterized by an unique polypeptide composition as well as by an unique velocity of transport; (3) the disappearance of individual labeled intra-axonally transported proteins from the axons and synaptic terminals with half-times ranging from several hours to more than 8 days.
The possibility that intra-axonal transport functions as a temporal mediator of alterations in axonal or synaptic events is discussed in relation to these findings.
Keywords: visual system, rabbit, gradient gel electrophoresis, autoradiography
Full text
PDF




Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bray J. J., Austin L. Axoplasmic transport of 14C proteins at two rates in chicken sciatic nerve. Brain Res. 1969 Jan;12(1):230–233. doi: 10.1016/0006-8993(69)90069-9. [DOI] [PubMed] [Google Scholar]
- Cuénod M., Schonbach J. Synaptic proteins and axonal flow in the pigeon visual pathway. J Neurochem. 1971 Jun;18(6):809–816. doi: 10.1111/j.1471-4159.1971.tb12010.x. [DOI] [PubMed] [Google Scholar]
- DROZ B., LEBLOND C. P. AXONAL MIGRATION OF PROTEINS IN THE CENTRAL NERVOUS SYSTEM AND PERIPHERAL NERVES AS SHOWN BY RADIOAUTOGRAPHY. J Comp Neurol. 1963 Dec;121:325–346. doi: 10.1002/cne.901210304. [DOI] [PubMed] [Google Scholar]
- Edström A., Mattsson H. Rapid axonal transport in vitro in the sciatic system of the frog of fucose-, glucosamine- and sulphate-containing material. J Neurochem. 1972 Jul;19(7):1717–1729. doi: 10.1111/j.1471-4159.1972.tb06216.x. [DOI] [PubMed] [Google Scholar]
- Elam J. S., Agranoff B. W. Transport of proteins and sulfated mucopolysaccharides in the goldfish visual system. J Neurobiol. 1971;2(4):379–390. doi: 10.1002/neu.480020409. [DOI] [PubMed] [Google Scholar]
- Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
- Giolli R. A., Guthrie M. D. The primary optic projections in the rabbit. An experimental degeneration study. J Comp Neurol. 1969 May;136(1):99–126. doi: 10.1002/cne.901360107. [DOI] [PubMed] [Google Scholar]
- Grafstein B., Murray M. Transport of protein in goldfish optic nerve during regeneration. Exp Neurol. 1969 Dec;25(4):494–508. doi: 10.1016/0014-4886(69)90093-4. [DOI] [PubMed] [Google Scholar]
- Hendrickson A. E., Cowan W. M. Changes in the rate of axoplasmic transport during postnatal development of the rabbit's optic nerve and tract. Exp Neurol. 1971 Mar;30(3):403–422. doi: 10.1016/0014-4886(71)90142-7. [DOI] [PubMed] [Google Scholar]
- Karlsson J. -O., Sjöstrand J. Electrophoretic characterization of rapidly transported proteins in axons of retinal ganglion cells. FEBS Lett. 1971 Sep 1;16(4):329–332. doi: 10.1016/0014-5793(71)80382-4. [DOI] [PubMed] [Google Scholar]
- Karlsson J. O., Sjöstrand J. Rapid intracellular transport of fucose-containing glycoproteins in retinal ganglion cells. J Neurochem. 1971 Nov;18(11):2209–2216. doi: 10.1111/j.1471-4159.1971.tb05079.x. [DOI] [PubMed] [Google Scholar]
- Karlsson J. O., Sjöstrand J. Synthesis, migration and turnover of protein in retinal ganglion cells. J Neurochem. 1971 May;18(5):749–767. doi: 10.1111/j.1471-4159.1971.tb12005.x. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Lasek R. J. Axonal transport of proteins in dorsal root ganglion cells of the growing cat: A comparison of growing and mature neurons. Brain Res. 1970 May 20;20(1):121–126. doi: 10.1016/0006-8993(70)90160-5. [DOI] [PubMed] [Google Scholar]
- Lasek R. J. Axoplasmic transport of labeled proteins in rat ventral motoneurons. Exp Neurol. 1968 May;21(1):41–51. doi: 10.1016/0014-4886(68)90032-0. [DOI] [PubMed] [Google Scholar]
- Lasek R. Axoplasmic transport in cat dorsal root ganglion cells: as studied with [3-H]-L-leucine. Brain Res. 1968 Mar;7(3):360–377. doi: 10.1016/0006-8993(68)90003-6. [DOI] [PubMed] [Google Scholar]
- Lux H. D., Schubert P., Kreutzberg G. W., Globus A. Excitation and axonal flow: autoradiographic study on motoneurons intracellularly injected with a 3H-amino acid. Exp Brain Res. 1970;10(2):197–204. doi: 10.1007/BF00234732. [DOI] [PubMed] [Google Scholar]
- Marko P., Susz J. -P., Cuénod M. Synaptosomal proteins and axoplasmic flow: Fractionation by SDS polyacrylamide gel electrophoresis. FEBS Lett. 1971 Oct 1;17(2):261–264. doi: 10.1016/0014-5793(71)80160-6. [DOI] [PubMed] [Google Scholar]
- McEwen B. S., Forman D. S., Grafstein B. Components of fast and slow axonal transport in the goldfish optic nerve. J Neurobiol. 1971;2(4):361–377. doi: 10.1002/neu.480020408. [DOI] [PubMed] [Google Scholar]
- Schonback J., Cuénod M. Axoplasmic migration of protein. A light microscopic autoradiographic study in the avian retino-tectal pathway. Exp Brain Res. 1971;12(3):275–282. doi: 10.1007/BF00237920. [DOI] [PubMed] [Google Scholar]
- Sjöstrand J., Karlsson J. O. Axoplasmic transport in the optic nerve and tract of the rabbit: a biochemical and radioautographic study. J Neurochem. 1969 Jun;16(3):833–844. doi: 10.1111/j.1471-4159.1969.tb08971.x. [DOI] [PubMed] [Google Scholar]
- Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]