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. 1974 Jun;71(6):2231–2235. doi: 10.1073/pnas.71.6.2231

Dominance of the Senescent Phenotype in Heterokaryons Between Replicative and Post-Replicative Human Fibroblast-Like Cells

Thomas H Norwood 1, William R Pendergrass *, Curtis A Sprague 1, George M Martin 1
PMCID: PMC388425  PMID: 4366757

Abstract

In heterokaryons between senescent and young diploid fibroblast-like cells, dominance of the former with respect to nuclear DNA synthesis (incorporation of [3H]thymidine) was demonstrated. For identification of the respective partners, double-layer autoradiography was used after the old cells were labeled with [3H]methionine and the young cells were labeled with [14C]thymidine. Synchrony of nuclear labeling (i.e., all nuclei in a cell labeled with [3H]thymidine) was observed in the majority of di- and polykaryons during the second and third of three 24-hr periods of labeling with [3H]thymidine. The results are compatible with either terminal differentiation or error theories of clonal senescence.

Keywords: cell aging, cell fusion, cell division, fibroblast cultures

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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