Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Jun;71(6):2377–2381. doi: 10.1073/pnas.71.6.2377

Regulation of Adenylate Kinase and Creatine Kinase Activities in Myogenic Cells

Helgi Tarikas 1, David Schubert 1
PMCID: PMC388458  PMID: 4366764

Abstract

The regulation of the specific activities of adenylate kinase (EC 2.7.4.3) and creatine kinase (EC 2.7.3.2) in myogenic cell lines is independent of cell fusion. The observed increases in enzyme specific activities are cell density dependent, and may be further broken down into contributions from an increase in enzyme activity per cell and a decrease in protein per cell. Only the former appears to be affected by medium conditioning.

Keywords: myogenesis, enzyme regulation

Full text

PDF
2377

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adelstein R. S., Conti M. A., Johnson G. S., Pastan I., Pollard T. D. Isolation and characterization of myosin from cloned mouse fibroblasts. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3693–3697. doi: 10.1073/pnas.69.12.3693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson E. C., Petersen D. F. Cell growth and division. II. Experimental studies of cell volume distributions in mammalian suspension cultures. Biophys J. 1967 Jul;7(4):353–364. doi: 10.1016/S0006-3495(67)86593-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coleman J. R., Coleman A. W. Muscle differentiation and macromolecular synthesis. J Cell Physiol. 1968 Oct;72(2 Suppl):19–34. doi: 10.1002/jcp.1040720404. [DOI] [PubMed] [Google Scholar]
  4. Fambrough D., Rash J. E. Development of acetylcholine sensitivity during myogenesis. Dev Biol. 1971 Sep;26(1):55–68. doi: 10.1016/0012-1606(71)90107-2. [DOI] [PubMed] [Google Scholar]
  5. Kidokoro Y. Development of action potentials in a clonal rat skeletal muscle cell line. Nat New Biol. 1973 Jan 31;241(109):158–159. doi: 10.1038/newbio241158a0. [DOI] [PubMed] [Google Scholar]
  6. Konigsberg I. R. Diffusion-mediated control of myoblast fusion. Dev Biol. 1971 Sep;26(1):133–152. doi: 10.1016/0012-1606(71)90113-8. [DOI] [PubMed] [Google Scholar]
  7. Layman D. L., McGoodwin E. B., Martin G. R. The nature of the collagen synthesized by cultured human fibroblasts. Proc Natl Acad Sci U S A. 1971 Feb;68(2):454–458. doi: 10.1073/pnas.68.2.454. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Meisler A. I. Studies on contract inhibition of growth in the mouse fibroblast, 3T3. I. Changes in cell size and composition during 'unrestricted' growth. J Cell Sci. 1973 May;12(3):847–859. doi: 10.1242/jcs.12.3.847. [DOI] [PubMed] [Google Scholar]
  9. Nelson P. G., Peacock J., Minna J. An active electrical response in fibroblasts. J Gen Physiol. 1972 Jul;60(1):58–71. doi: 10.1085/jgp.60.1.58. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. O'Neill M. C., Stockdale F. E. Differentiation without cell division in cultured skeletal muscle. Dev Biol. 1972 Dec;29(4):410–418. doi: 10.1016/0012-1606(72)90081-4. [DOI] [PubMed] [Google Scholar]
  11. Paterson B., Prives J. Appearance of acetylcholine receptor in differentiating cultures of embryonic chick breast muscle. J Cell Biol. 1973 Oct;59(1):241–245. doi: 10.1083/jcb.59.1.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Patrick J., Heinemann S. F., Lindstrom J., Schubert D., Steinbach J. H. Appearance of acetylcholine receptors during differentiation of a myogenic cell line. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2762–2766. doi: 10.1073/pnas.69.10.2762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. REPORTER M. C., KONIGSBERG I. R., STREHLER B. L. Kinetics of accumulation of creatine phosphokinase activity in developing embryonic skeletal muscle in vivo and in monolayer culture. Exp Cell Res. 1963 Apr;30:410–417. doi: 10.1016/0014-4827(63)90313-6. [DOI] [PubMed] [Google Scholar]
  14. Richler C., Yaffe D. The in vitro cultivation and differentiation capacities of myogenic cell lines. Dev Biol. 1970 Sep;23(1):1–22. doi: 10.1016/s0012-1606(70)80004-5. [DOI] [PubMed] [Google Scholar]
  15. Ross R., Klebanoff S. J. The smooth muscle cell. I. In vivo synthesis of connective tissue proteins. J Cell Biol. 1971 Jul;50(1):159–171. doi: 10.1083/jcb.50.1.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schubert D., Tarikas H., Harris A. J., Heinemann S. Induction of acetylcholine esterase activity in a mouse neuroblastoma. Nat New Biol. 1971 Sep 15;233(37):79–80. doi: 10.1038/newbio233079a0. [DOI] [PubMed] [Google Scholar]
  17. Schubert D., Tarikas H., Humphreys S., Heinemann S., Patrick J. Protein synthesis and secretion in a myogenic cell line. Dev Biol. 1973 Jul;33(1):18–37. doi: 10.1016/0012-1606(73)90161-9. [DOI] [PubMed] [Google Scholar]
  18. Shainberg A., Yagil G., Yaffe D. Alterations of enzymatic activities during muscle differentiation in vitro. Dev Biol. 1971 May;25(1):1–29. doi: 10.1016/0012-1606(71)90017-0. [DOI] [PubMed] [Google Scholar]
  19. Shainberg A., Yagil G., Yaffe D. Control of myogenesis in vitro by Ca 2 + concentration in nutritional medium. Exp Cell Res. 1969 Nov;58(1):163–167. doi: 10.1016/0014-4827(69)90127-x. [DOI] [PubMed] [Google Scholar]
  20. VOGT M., DULBECCO R. Steps in the neoplastic transformation of hamster embryo cells by polyoma virus. Proc Natl Acad Sci U S A. 1963 Feb 15;49:171–179. doi: 10.1073/pnas.49.2.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Vogel Z., Sytkowski A. J., Nirenberg M. W. Acetylcholine receptors of muscle grown in vitro. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3180–3184. doi: 10.1073/pnas.69.11.3180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. White N. K., Hauschka S. D. Muscle development in vitro. A new conditioned medium effect on colony differentiation. Exp Cell Res. 1971 Aug;67(2):479–482. doi: 10.1016/0014-4827(71)90437-x. [DOI] [PubMed] [Google Scholar]
  23. Yaffe D. Retention of differentiation potentialities during prolonged cultivation of myogenic cells. Proc Natl Acad Sci U S A. 1968 Oct;61(2):477–483. doi: 10.1073/pnas.61.2.477. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES