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. 1974 Jun;71(6):2432–2434. doi: 10.1073/pnas.71.6.2432

Enhancement of DNA Synthesis in a Mammalian Cell-Free System by Trypsin Treatment

Ronald L Brown 1,2,3, Elton Stubblefield 1,2,3
PMCID: PMC388471  PMID: 4526218

Abstract

DNA synthesis in a broken cellular preparation of Chinese hamster cells was enhanced approximately 10-fold by a brief trypsin treatment. Alphachymotrypsin also enhanced the synthesis, whereas Pronase did not. The trypsin appears to be acting on a component of the nucleus. Evidence suggests that the trypsin is not removing protein from the DNA, but may be activating the system some other way.

Keywords: Chinese hamster cells

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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