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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Jun;71(6):2487–2490. doi: 10.1073/pnas.71.6.2487

Phosphate Metabolism in Intact Human Erythrocytes: Determination by Phosphorus-31 Nuclear Magnetic Resonance Spectroscopy

Thomas O Henderson 1,2,*, Anthony J R Costello 1,2, Akira Omachi 1,2
PMCID: PMC388484  PMID: 4366770

Abstract

Whole human blood was examined by 31P nuclear magnetic resonance spectroscopy. Individual phosphates (α,β,γ) of ATP were identifiable, and two microenvironments appeared to be present for this molecule. When sequential recordings of freshly collected blood were made, 2,3-diphosphoglycerate was observed to decrease in association with a concomitant increase in inorganic orthophosphate. When aged cells containing little 2,3-diphosphoglycerate were incubated in the presence of inosine and pyruvate, 2,3-diphosphoglycerate formation could be demonstrated. These results show that cellular metabolism can be recorded directly in intact cells by 31P nuclear magnetic resonance.

Keywords: phosphorylated compounds, cold storage, blood

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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