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. 1974 Jul;71(7):2610–2613. doi: 10.1073/pnas.71.7.2610

The Relationship of Gamone to the Mating Reaction in Chlamydomonas moewusii

Robert J McLean *, Carmen J Laurendi *, R Malcolm Brown Jr †
PMCID: PMC388515  PMID: 4528056

Abstract

Gamete-gamete adhesion in Chlamydomonas has long been attributed to large glycoprotein particles (gamone) on the flagellar membrane surface. These macromolecules may be incidentally sloughed off or released into the medium. Gamone isolated from the medium of one mating type will cause isoagglutination of opposite gametes. Our study of Chlamydomonas moewusii shows that such particles isolated from the cell medium are really membrane vesicles with mastigonemes (flagellar appendages) attached. The vesicles appear to bleb off the flagellar membrane as a normal process. Purified mastigonemes have no isoagglutinating activity, while purified membrane vesicles (with no mastigonemes attached) retain activity. Therefore, the large particles isolated by others from the cell medium of Chlamydomonas and thought to be equivalent to the gametic adhesion substance are in fact membrane vesicles upon which the adhesion sites are located, and these sites are probably much smaller than was previously supposed. Mastigonemes appear to have no role in gamete adhesion.

Keywords: gamete-gamete contact, cell surfaces, negative-staining, differential and density gradient centrifugation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S., Behrens U., Orner R., Haines T. H. Ultrastructure of intracellular and extracellular vesicles, membranes, and myelin figures produced by Ochromonas danica. J Ultrastruct Res. 1971 Jun;35(5):418–430. doi: 10.1016/s0022-5320(71)80003-5. [DOI] [PubMed] [Google Scholar]
  2. Bosmann H. B. Platelet adhesiveness and aggregation: the collagen:glycosyl, polypeptide:N-acetylgalactosaminyl and glycoprotein:galactosyl transferases of human platelets. Biochem Biophys Res Commun. 1971 Jun 4;43(5):1118–1124. doi: 10.1016/0006-291x(71)90578-x. [DOI] [PubMed] [Google Scholar]
  3. Bouck G. B. The structure, origin, isolation, and composition of the tubular mastigonemes of the Ochromas flagellum. J Cell Biol. 1971 Aug;50(2):362–384. doi: 10.1083/jcb.50.2.362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Keenan T. W., Morré D. J., Basu S. Ganglioside biosynthesis. Concentration of glycosphingolipid glycosyltransferases in Golgi apparatus from rat liver. J Biol Chem. 1974 Jan 10;249(1):310–315. [PubMed] [Google Scholar]
  5. Nicolson G. L., Painter R. G. Anionic sites of human erythrocyte membranes. II. Antispectrin-induced transmembrane aggregation of the binding sites for positively charged colloidal particles. J Cell Biol. 1973 Nov;59(2 Pt 1):395–406. doi: 10.1083/jcb.59.2.395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Nicolson G. L., Singer S. J. Ferritin-conjugated plant agglutinins as specific saccharide stains for electron microscopy: application to saccharides bound to cell membranes. Proc Natl Acad Sci U S A. 1971 May;68(5):942–945. doi: 10.1073/pnas.68.5.942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nicolson G. L. Temperature-dependent mobility of concanavalin A sites on tumour cell surfaces. Nat New Biol. 1973 Jun 13;243(128):218–220. doi: 10.1038/newbio243218a0. [DOI] [PubMed] [Google Scholar]
  8. Ringo D. L. Flagellar motion and fine structure of the flagellar apparatus in Chlamydomonas. J Cell Biol. 1967 Jun;33(3):543–571. doi: 10.1083/jcb.33.3.543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Roseman S. The synthesis of complex carbohydrates by multiglycosyltransferase systems and their potential function in intercellular adhesion. Chem Phys Lipids. 1970 Oct;5(1):270–297. doi: 10.1016/0009-3084(70)90024-1. [DOI] [PubMed] [Google Scholar]
  10. Roth S., McGuire E. J., Roseman S. Evidence for cell-surface glycosyltransferases. Their potential role in cellular recognition. J Cell Biol. 1971 Nov;51(21):536–547. doi: 10.1083/jcb.51.2.536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Roth S., White D. Intercellular contact and cell-surface galactosyl transferase activity (cell culture-mouse-radioautography-contact inhibition-cis-and trans-galactosylation). Proc Natl Acad Sci U S A. 1972 Feb;69(2):485–489. doi: 10.1073/pnas.69.2.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schmeisser E. T., Baumgartel D. M., Howell S. H. Gametic differentiation in Chlamydomonas reinhardi: cell cycle dependency and rates in attainment of mating competency. Dev Biol. 1973 Mar;31(1):31–37. doi: 10.1016/0012-1606(73)90318-7. [DOI] [PubMed] [Google Scholar]
  13. Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]
  14. Witman G. B., Carlson K., Berliner J., Rosenbaum J. L. Chlamydomonas flagella. I. Isolation and electrophoretic analysis of microtubules, matrix, membranes, and mastigonemes. J Cell Biol. 1972 Sep;54(3):507–539. doi: 10.1083/jcb.54.3.507. [DOI] [PMC free article] [PubMed] [Google Scholar]

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