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. 1974 Jul;71(7):2675–2678. doi: 10.1073/pnas.71.7.2675

Fusion and Compatibility of Camphor and Octane Plasmids in Pseudomonas

George I N Chou 1,*, Dvorah Katz 1, I C Gunsalus 1
PMCID: PMC388530  PMID: 4527812

Abstract

The octane (OCT) plasmid in Pseudomonas putida derived from the ω-hydroxylase-carrying strain of Coon and coworkers is transferable to the camphor (CAM) plasmid-bearing strain by conjugation or by transduction. While the majority of the Cam +Oct+ exconjugants segregate Cam+ or Oct+ cells, exconjugants with stable Cam +Oct+ phenotype (CAM-OCT) can be detected at a low frequency. The transductants are all of the CAM-OCT phenotype. In the stable Cam +Oct+ strains, the OCT plasmid resembles the CAM plasmid with respect to curing by mitomycin C, transfer in conjugation, and reaction to ts (temperature-sensitive) mutation specifically affecting CAM plasmid replication. Therefore, it is suggested that certain regions of homology exist between the CAM and OCT plasmids that enable them to recombine to form a single plasmid, and to overcome the incompatibility barrier that prevents their coexisting.

Keywords: plasmid transduction, recombination

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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