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. 1974 Jul;71(7):2736–2739. doi: 10.1073/pnas.71.7.2736

Formation of 10-Formylfolic Acid, a Potent Inhibitor of Dihydrofolate Reductase, in Rat Liver Slices Incubated with Folic Acid

M d'Urso-Scott 1,2, J Uhoch 1,2, J R Bertino 1,2
PMCID: PMC388544  PMID: 4527808

Abstract

During investigation of folate polyglutamate biosynthesis in rat liver slices utilizing [2-14C]folic acid, a folate compound that behaved like a polyglutamate form in the Sephadex G-15 gel filtration system was found to accumulate. Subsequent chromatographic, spectral, chemical, and enzymic studies have indicated that the compound formed in liver slices incubated with [14C]folic acid with and without methotrexate was 10-formyl folate. This folate is of interest in that it is the most potent natural inhibitor of dihydrofolate reductase known and may be capable of serving a regulatory function within the cell.

Keywords: folate metabolism, folate coenzymes, natural folate enzyme inhibitors, regulation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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