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. 1974 Jul;71(7):2800–2802. doi: 10.1073/pnas.71.7.2800

Antibodies to Yeast Phenylalanine Transfer Ribonucleic Acid Are Specific for the Odd Nucleoside Y in the Anticodon Loop

Sara Fuchs *, Aharon Aharonov *, Michael Sela *, Friedrich Von Der Haar †, Friedrich Cramer †
PMCID: PMC388559  PMID: 4527666

Abstract

Antibodies with specificity to a single species of tRNA were elicited in a goat by immunization with a glutaraldehyde conjugate of yeast phenylalanine transfer RNA with bovine gamma globulin. The specificity of the antibodies was studied by a radioimmunoassay measuring the direct binding of [3H]tRNAPhe or the inhibition of the binding. The antibodies formed are predominantly directed towards the characteristic highly modified nucleoside Y, which is located right next to the anticodon. The antibodies bind specifically to tRNAPhe, to oligonucleotides derived by enzymatic digestion from the anticodon loop of tRNAPhe, and to the Y nucleoside itself. tRNA species which do not contain Y in their sequences, or tRNAPhe from which the Y base has been excised, do not bind to the antibodies. Yeast tRNAPhe can be separated from other tRNA species with an immunoadsorbent of antibodies to tRNAPhe.

Keywords: Y base, tRNA fractionation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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