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. 1974 Jul;71(7):2863–2866. doi: 10.1073/pnas.71.7.2863

The Effects of Hemin and Double-Stranded RNA on α and β Globin Synthesis in Reticulocyte and Krebs II Ascites Cell-Free Systems and the Relationship of These Effects to an Initiation Factor Preparation

Yves Beuzard 1,*, Irving M London 1
PMCID: PMC388572  PMID: 4528029

Abstract

Protein synthesis in reticulocyte lysates ceases abruptly in the absence of added hemin or in the presence of double-stranded RNA. A similar effect of double-stranded RNA is observed in Krebs II ascites cell-free systems translating exogenous globin mRNA. The shut-off of protein synthesis is due to inhibition of initiation and can be prevented or reversed by addition of the initiation factor preparation M3. Preparations of M1, M2, and dissociation factor are ineffective under these conditions. The effects of added hemin, M3, and globin mRNA on the synthesis of α and β globin chains have been studied in the reticulocyte and ascites cell extracts. When the concentration of M3 is rate limiting, the synthesis of β chains exceeds that of α chains. When the concentration of mRNA is rate limiting, synthesis of α and β chains is more nearly equal.

Keywords: translational control, initiation factor preparation M3, α:β globin chain ratio

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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