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. 1974 Jul;71(7):2882–2886. doi: 10.1073/pnas.71.7.2882

A Cleavage Map of Bacteriophage ϕX174 Genome

Amy Shiu Lee 1, Robert L Sinsheimer 1
PMCID: PMC388576  PMID: 4527604

Abstract

Restriction endonucleases isolated from Hemophilus influenzae, Hemophilus parainfluenzae, and Hemophilus aegyptius were used to cleave ϕX174 replicative form DNA into three sets of specific DNA fragments. The order of these fragments in the ϕX replicative form molecule was determined by (1) analysis of partial digest products, (2) analysis of overlapping sets of fragments produced by two different restrictive enzymes. On the basis of these results, a detailed physical map of the ϕX174 genome has been constructed with respect to the cleavage sites of all three enzymes.

Keywords: restriction endonuclease, DNA fragments, DNA mapping, gel electrophoresis

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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