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. 1974 Aug;71(8):3054–3058. doi: 10.1073/pnas.71.8.3054

A DNA-Protein Complex Involved in Bacteriophage ϕX174 Particle Formation

Peter J Weisbeek 1,*, Robert L Sinsheimer 1
PMCID: PMC388619  PMID: 4528401

Abstract

A ϕX-specific DNA-protein complex has been isolated from ϕX-infected cells. This complex contains infective circular single-stranded DNA, the proteins of the genes F, G, H, and J in the same proportions as in the phage particle, and, in addition, the gene D-protein. The D-protein makes up 12-22% of the protein part of the complex. The sedimentation value of the complex is about 140 S. In vitro, the complex can be converted to the normal 114S phage particle (with a concomitant loss of its D-protein) or to an uninfective 70S particle and a small amount of free single-stranded DNA. The fast sedimenting particle is not associated with membranes.

Keywords: intermediates, assembly, gene D-protein

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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