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. 1974 Aug;71(8):3087–3090. doi: 10.1073/pnas.71.8.3087

5′-Guanylylimidodiphosphate, A Potent Activator of Adenylate Cyclase Systems in Eukaryotic Cells

Constantine Londos *, Yoram Salomon *, Michael C Lin *, James P Harwood *, Michael Schramm *, J Wolff †, Martin Rodbell *
PMCID: PMC388626  PMID: 4607368

Abstract

5′-Guanylylimidodiphosphate (Gpp(NH)-p) stimulates adenylate cyclase [ATP-pyrophosphate-lyase (cyclizing), EC 4.6.1.1] activity in plasma membranes isolated from frog and salmon erythrocytes, from rat adrenal, hepatic, and fat cells, and from bovine thyroid cells. The nucleotide acts cooperatively with the various hormones (glucagon, secretin, ACTH, thyrotropin, and catecholamines) that stimulate these adenylate cyclase systems with resultant activities that equal or exceed those obtained with hormone plus GTP or with fluoride ion. In the absence of hormones, Gpp(NH)p is a considerably more effective activator than GTP, and, under certain conditions of incubation, stimulates rat fat cell adenylate cyclase to levels of activity (about 20 nmoles of 3′,5′-adenosine monophosphate mg protein per min) far higher than reported hitherto for any adenylate cyclase system examined. The nucleotide activates frog erythrocyte adenylate cyclase when the catecholamine receptor is blocked by the competitive antagonist, propranolol, and activates the enzyme from an adrenal tumor cell line which lacks functional ACTH receptors. In contrast, Gpp(NH)p does not stimulate adenylate cyclase in extracts from Escherichia coli B. Gpp(NH)p appears to be a useful probe for investigating the mechanism of hormone and nucleotide action on adenylate cyclase systems in eukaryotic cells.

Keywords: hormones, membranes, cyclic AMP, guanine nucleotides, allosteric regulation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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