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. 1974 Aug;71(8):3134–3138. doi: 10.1073/pnas.71.8.3134

Interaction of the Operator of the Tryptophan Operon with Repressor

John K Rose 1, Charles Yanofsky 1
PMCID: PMC388636  PMID: 4528605

Abstract

Transcription studies in vitro on repression of the tryptophan operon of Escherichia coli show that partially purified trp repressor binds specifically to DNA containing the trp operator with a repressor-operator dissociation constant of about 0.2 nM in 0.12 M salt at 37°, a value consistent with the extent of trp operon regulation in vivo. The half-life of the trp repressor-trp operator complex is less than 2 min in vitro in 0.12 M salt.

Keywords: repressor-operator interactions, trp repressor, in vitro transcription, Escherichia coli

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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