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. 1974 Aug;71(8):3204–3207. doi: 10.1073/pnas.71.8.3204

Methylated Messenger RNA Synthesis In Vitro by Purified Reovirus

Aaron J Shatkin 1
PMCID: PMC388651  PMID: 4528644

Abstract

Purified human reovirus contains RNA methylase activity in addition to an RNA polymerase. Virions incubated under appropriate conditions in the presence of S-adenosyl-L-methionine synthesize mRNA that is specifically methylated in the 5′-terminal guanosine. Alkaline digestion of the methylated RNA released a 5′-terminal dinucleotide, ppG′pCp, indicating that the guanosine contains 2′-O-methylribose. The possible roles of methylation in viral and cellular mRNA function is discussed.

Keywords: methylation, RNA virus

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banerjee A. K., Shatkin A. J. Transcription in vitro by reovirus-associated ribonucleic acid-dependent polymerase. J Virol. 1970 Jul;6(1):1–11. doi: 10.1128/jvi.6.1.1-11.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Banerjee A. K., Ward R., Shatkin A. J. Initiation of reovirus mRNA synthesis in vitro. Nat New Biol. 1971 Apr 7;230(14):169–172. doi: 10.1038/newbio230169a0. [DOI] [PubMed] [Google Scholar]
  3. Borsa J., Graham A. F. Reovirus: RNA polymerase activity in purified virions. Biochem Biophys Res Commun. 1968 Dec 30;33(6):895–901. doi: 10.1016/0006-291x(68)90396-3. [DOI] [PubMed] [Google Scholar]
  4. Furuichi Y. "Methylation-coupled" transcription by virus-associated transcriptase of cytoplasmic polyhedrosis virus containing double-stranded RNA. Nucleic Acids Res. 1974 Jun;1(6):809–822. doi: 10.1093/nar/1.6.809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Graziadei W. D., 3rd, Roy D., Konigsberg W., Lengyel P. Translation of reovirus messenger ribonucleic acids synthesized in vitro into reovirus proteins in a mouse L cell extract. Arch Biochem Biophys. 1973 Sep;158(1):266–275. doi: 10.1016/0003-9861(73)90621-8. [DOI] [PubMed] [Google Scholar]
  6. Hay A. J., Joklik W. K. Demonstration that the same strand of reovirus genome RNA is transcribed in vitro and in vivo. Virology. 1971 May;44(2):450–453. doi: 10.1016/0042-6822(71)90276-5. [DOI] [PubMed] [Google Scholar]
  7. Levin D. H., Mendelsohn N., Schonberg M., Klett H., Silverstein S., Kapuler A. M., Acs G. Properties of RNA transcriptase in reovirus subviral particles. Proc Natl Acad Sci U S A. 1970 Jul;66(3):890–897. doi: 10.1073/pnas.66.3.890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McDowell M. J., Joklik W. K., Villa-Komaroff L., Lodish H. F. Translation of reovirus messenger RNAs synthetesized in vitro into reovirus polypeptides by several mammalian cell-free extracts. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2649–2653. doi: 10.1073/pnas.69.9.2649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Miura K., Watanabe K., Sugiura M. 5'-Terminal nucleotide sequences of the double-stranded RNA of silkworm cytoplasmic polyhedrosis virus. J Mol Biol. 1974 Jun 15;86(1):31–48. doi: 10.1016/s0022-2836(74)80005-7. [DOI] [PubMed] [Google Scholar]
  10. Roberts B. E., Paterson B. M. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330–2334. doi: 10.1073/pnas.70.8.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schonberg M., Silverstein S. C., Levin D. H., Acs G. Asynchronous synthesis of the complementary strands of the reovirus genome. Proc Natl Acad Sci U S A. 1971 Feb;68(2):505–508. doi: 10.1073/pnas.68.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shatkin A. J., Sipe J. D., Loh P. Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis. J Virol. 1968 Oct;2(10):986–991. doi: 10.1128/jvi.2.10.986-991.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shatkin A. J., Sipe J. D. RNA polymerase activity in purified reoviruses. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1462–1469. doi: 10.1073/pnas.61.4.1462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shimotono K., Miura K. 5'-Terminal structure of messenger RNA transcribed by RNA polymerase of silkworm cytoplasmic polyhedrosis virus containing double-stranded RNA. J Mol Biol. 1974 Jun 15;86(1):21–30. doi: 10.1016/s0022-2836(74)80004-5. [DOI] [PubMed] [Google Scholar]
  15. Skehel J. J., Joklik W. K. Studies on the in vitro transcription of reovirus RNA catalyzed by reovirus cores. Virology. 1969 Dec;39(4):822–831. doi: 10.1016/0042-6822(69)90019-1. [DOI] [PubMed] [Google Scholar]
  16. Smith R. E., Zweerink H. J., Joklik W. K. Polypeptide components of virions, top component and cores of reovirus type 3. Virology. 1969 Dec;39(4):791–810. doi: 10.1016/0042-6822(69)90017-8. [DOI] [PubMed] [Google Scholar]

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