Skip to main content
. 2013 Feb 22;66(1):133–147. doi: 10.1007/s10616-013-9545-7

Table 2.

ATCC cell lines and growth media

Cell line ATCC # Growth medium Supplements
NIH3T3 CRL-1658 DMEM 10 % FBS
L929 CCL-1 EMEM 10 % FBS
MC3T3-E1, subclone 4 CRL-2593 MEM 10 % FBS
RAW 264.7 TIB-71 DMEM 10 % FBS
M. dunni CRL-2017 McCoy’s 5A 10 % FBS
P3X63Ag8.653 CRL-1580 RPMI-1640 10 % FBS
HK-PEG-1 CCL-189 IMDM 20 % FBS
Vero CCL-81 MEM- α 10 % FBS
COS-7 CRL-1651 DMEM 10 % FBS
HEPM CRL-1486 EMEM 10 % FBS
SK-BR-3 HTB-30 McCoy’s 5A 10 % FBS
MCF 10A CRL-10317 MECGM 0.4 % bovine pituitary extract, 0.5 μg/mL hydrocortisone, 3 ng/mL human epidermal growth factor, 5 μg/mL insulin
HeLa CCL-2 EMEM 10 % FBS
CHO-K1 CCL-61 F-12 K 10 % FBS

Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC), Eagle’s Minimum Essential Medium (EMEM) (ATCC), Minimum Essential Medium (MEM) (Gibco, Carlsbad, CA, USA), Iscove’s Modified Dulbecco’s Medium (IMDM) (ATCC), MEM- α (Invitrogen, Carslbad, CA, USA), RPMI-1640 (ATCC), McCoy’s 5A (ATCC), F-12 K Medium (ATCC), Mammary Epithelial Cell Growth Medium (MECGM) and associated supplements (Lonza, Rockland, ME, USA), fetal bovine serum (FBS) (Invitrogen)