Table 3.

Sequencing primers

STR marker	Primers (5′–3′)	Amplicon size (bp)	Ta (°C)
18-3	18-3 F:TCTTTCTCCTTTTGTGTCATGC 18-3 R: GTCAAAGTTGGGGTTACAGAATG*	281–313	54
4-2	4-2 F:AAGCTTCTCTGGCCATTTGA 4-2 R:GTTCATAAACTTCAAGCAATGACA	217–248	57
6-7	6-7 F:AGTCCACCCAGTGCATTCTC 6-7 R:GCATGTGGCTGGTATGCTGTT	333–515	60
9-2	9-2 F:GGCTCTCTCACACCTCATCC* 9-2 R:GTCCATGAATCCAGACATTCC	318–360	60
15-3	15-3F: TCTGGGCGTGTCTGTCATAA 15-3 R:GTTCTCAGGGAGGAGTGTGCT	157–222	60
6-4	6-4 F:TTTGCAACAGCTCAGTTTCC 6-4 R:GAATCGCTGGCAGATCTTAGG	276–311	52
12-1	12-1 F:CAAAATTGTCATTGAACACATGTAA* 12-1 R:GCAATGGTCAAGAAATACTGAAGTACAA	222–259	55
5-5	5-5 F:CGTTTTACCTGGCTGACACA 5-5 R:GATGCTTGCCTGTTCCTAGC	258–298	60
X-1	X-1 F:GGATGGATGGATGGATGAAA X-1 R:GAAGGTATATATCAAGATGGCATTATCA	357–442	54

Sequencing primers listed with their respective amplicon size range (bp) and annealing temperatures (T_a). Primers that are not included in the multiplex assay (*) were designed for samples that were difficult to sequence with the original primers