Abstract
The tetranucleotide TpΨpCpGp, when bound to Escherichia coli 50S ribosomal subunits, can replace intact tRNA in the stringent factor-directed synthesis of guanosine tetra- and pentaphosphates. The TpΨpCpGp-dependent fragment reaction has a strict requirement for the 50S and 30S ribosomal subunits and synthetic or natural messenger RNA.
Keywords: stringent factor, puromycin, 3′-terminus of tRNA, Escherichia coli
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