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. 1974 Aug;71(8):3278–3282. doi: 10.1073/pnas.71.8.3278

Specificity of the DNA Product of RNA-Dependent DNA Polymerase in Type C Viruses: III. Analysis of Viruses Derived from Syrian Hamsters*

Hiromi Okabe 1, Raymond V Gilden 1, Masakazu Hatanaka 1
PMCID: PMC388668  PMID: 4137938

Abstract

DNA transcripts were prepared from three related viruses of hamster and analyzed by interviral hybridization and by reaction with cellular DNA. A virus (G-HaLV), isolated from a dimethylbenzanthraceneinduced tumor cell in Graffi hamsters, contained nucleic acid sequences highly specific for hamster cell DNA and did not react with mouse cell DNA nor did its transcript show homology (<5%) with mouse or rat viral RNAs. The hamster-specific sarcoma virus, B-34, isolated by Bassin and coworkers from tumors induced by the Harvey strain of murine sarcoma virus, contained mouse-, hamster-, and possibly rat-specific sequences. B-34 transcripts were predominantly mouse-specific. GLOH-, a lymphomagenic virus derived by dilution beyond the transforming endpoint of a hamster-specific sarcoma virus obtained from tumors induced by the Gross pseudotype of murine sarcoma virus, also contained hamster- and mouse-specific sequences. Only a portion of its hamster sequence (about 50%) was shared with B-34 and G-HaLV viruses. As expected, transcripts of GLOH- virus were reactive with mouse and hamster cellular DNA.

Keywords: nucleic acid hybridization, hamster-mouse pseudotypes, viral sequences in cellular DNA

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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