Abstract
Here, we report the draft genome sequence of Clostridium mangenotii strain TR, which was isolated from the fecal material of a timber rattlesnake. This bacterium is nonpathogenic but contains 68 genes involved in virulence, disease, and defense.
GENOME ANNOUNCEMENT
Clostridium mangenotii is a Gram-positive rod that is capable of forming endospores. It is nonmotile and is a strict anaerobe, with an optimal growth temperature of 37°C. This bacterium is found in soil and has been isolated from African soil in particular (1). In this study, C. mangenotii strain TR was isolated from the fecal material of a timber rattlesnake using the ethanol shock method (2). The cells were plated on blood agar and incubated for 48 h under anaerobic conditions.
A colony of C. mangenotii was sent to Molecular Research LP (MR DNA [http://www.mrdnalab.com]). DNA was isolated using the Qiagen mini DNA isolation kit (Qiagen, Inc.). Afterward, using an Ion Express library kit and the Ion Torrent PGM sequencer (Life Technologies), a draft genome was sequenced according to the manufacturer’s guidelines. Using NGen (DNAStar), the genome was assembled, and annotation of the genome was performed using RAST (http://rast.nmpdr.org) (3).
In total, 82 contigs with protein-encoding genes (PEGs) were constructed, totaling 3,052,440 bp. Annotation in RAST revealed 146 RNAs, 3,093 coding sequences, and 323 subsystems. There were 68 genes involved in virulence, disease, and defense. Some of these genes are involved in vancomycin, fluoroquinolone, zinc, and arsenic resistance.
Nucleotide sequence accession numbers.
This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession no. AXUS00000000. The version described in this paper is version AXUS01000000.
ACKNOWLEDGMENTS
We thank Bryan Albrecht, Zina Haywood, Stephanie Sklba, Donald Zakutansky, and Jennifer Charpentier for their enthusiastic support of this research. We also thank the following students for their aid in isolating the bacterium: Elias Bychowski, Danielle Carlson, Cathryn Ellis, Catherine Harvey, Vitaliy Kuzmych, Erika McCullough, Jennifer McGrath, Mary O’Connor, Dipal Patel, Amber Quintanilla, Thomas Smith, Yolanda Talley, and Lakendra Taylor.
This project was supported by funding provided by Gateway Technical College. Access to the timber rattlesnake specimen was authorized through a permit from the Minnesota Department of Natural Resources to P.A.C.
Footnotes
Citation McLaughlin RW, Cochran PA, Dowd SE, Andersen K, Anderson N, Brennan R, Brook N, Callaway T, Diamante K, Duberstine A, Fitch K, Freiheit H, Godlewski C, Gorman K, Haubrich M, Hernandez M, Hirtreiter A, Ivanoski B, Jaminet X, Kirkpatrick T, Kratowicz J, Latus C, Leable T, Lingafelt N, Lowe D, Lowrance H, Malsack L, Mazurkiewicz J, Merlos P, Messley J, Montemurro D, Nakitare S, Nelson C, Nye A, Pazera V, Pierangeli G, Rellora A, Reyes A, Roberts J, Robins S, Robinson J, Schultz A, Seifert S, Sigler E, Spangler J, Swift E, TenCate R, Thurber J, Vallee K, Wamboldt J, Whitten S, Woods D, Wright A, Yankunas D. 2014. Draft genome sequence of Clostridium mangenotii TR, isolated from the fecal material of a timber rattlesnake. Genome Announc. 2(1):e01107-13. doi:10.1128/genomeA.01107-13.
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