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. 1975 Nov;72(11):4366–4370. doi: 10.1073/pnas.72.11.4366

Regulation of synthesis of hepatic fatty acid synthetase: polysomal translation in a cell-free system.

A W Strauss, A W Alberts, S Hennessy, P R Vagelos
PMCID: PMC388722  PMID: 1060113

Abstract

Polysomes were isolated from livers of rats fed various diets and were translated in a protein-synthesizing system derived from cultured Chang liver cells. One of the labeled products was identified as complete subunit(s) of fatty acid synthetase by indirect immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the solubilized immunoprecipitate. The relative amounts of fatty acid synthetase synthesized by polysomes from livers of rats fed a normal diet, starved rats, and rats starved and refed a fat-free diet for 16 hr were 1, 0.1, and 10, respectively. Induction of synthesis of fatty acid synthetase after fat-free refeeding of starved rats began by 2 hr (3-fold increase over starved animals), was increasing rapidly by 5 hr (19-fold over starved animals), and reached a high level by 16 hr (95-fold over starved and 10-fold over normally fed).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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