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. 1975 Nov;72(11):4445–4449. doi: 10.1073/pnas.72.11.4445

Adenovirus-2 mRNA is transcribed as part of a high-molecular-weight precursor RNA.

S Bachenheimer, J E Darnell
PMCID: PMC388738  PMID: 1060124

Abstract

The order of transcription and the length of nascent RNA transcripts from adenovirus-2 (Ad-2) DNA in the nucleus of infected cells has been deduced by labeling the growing RNA chains in vivo for a very brief period, separating the RNA on the basis of size, and hybridizine to the ordered EcoRI restriction endonuclease fragments derived from Ad-2 DNA. The majority of the virus-specific RNA molecules are synthesized as very high-molecular-weight units beginning at a common point at least 25-30,000 base pairs from one end of the Ad-2 DNA. These molecules can be reduced in size without further RNA synthesis. The experiments indicate the obligatory origin of Ad-2 mRNA from a high-molecular-weight precursor molecule.

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Selected References

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