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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Nov;72(11):4501–4505. doi: 10.1073/pnas.72.11.4501

Cytochemical study of secretory process in transplantable insulinoma of syrian golden hamster.

A B Novikoff, A Yam, P M Novikoff
PMCID: PMC388750  PMID: 172911

Abstract

Electron microscopy, including phosphatase cytochemistry, indicates that the secretory granules of an insulinoma producing proinsulin and insulin are packaged by the endoplasmic reticulum (ER) and especially by a specialized region of ER which we call GERL because of the spatial relationship of this region to the Golgi apparatus and its apparent role in producing lysosomes. The granules are not derived from the Golgi apparatus. Preliminary evidence suggests this may be true also of pancreatic beta-cells.

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Selected References

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  1. Boutry J. M., Nivikoff A. B. Cytochemical studies on golgi apparatus, GERL, and lysosomes in neurons of dorsal root ganglia in mice. Proc Natl Acad Sci U S A. 1975 Feb;72(2):508–512. doi: 10.1073/pnas.72.2.508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. KIRKMAN H. A preliminary report concerning tumors observed in Syrian hamsters. Stanford Med Bull. 1962 Nov;20:163–166. [PubMed] [Google Scholar]
  3. Lazarus S. S., Volk B. W., Barden H. Localization of acid phosphatase activity and secretion mechanism in rabbit pancreatic B-cells. J Histochem Cytochem. 1966 Mar;14(3):233–246. doi: 10.1177/14.3.233. [DOI] [PubMed] [Google Scholar]
  4. Miller F., Herzog V. Die Lokalisation von Peroxydase und saurer Phosphatase in eosinophilen Leukocyten während der Reifung. Elek. Elektronenmikroskopisch-cytochemische Untersuchungen am Knochenmark von Ratte und Kaninchen. Z Zellforsch Mikrosk Anat. 1969;97(1):84–110. [PubMed] [Google Scholar]
  5. NOVIKOFF A. B., ESSNER E. Pathological changes in cytoplasmic organelles. Fed Proc. 1962 Nov-Dec;21:1130–1142. [PubMed] [Google Scholar]
  6. Novikoff A. B., Novikoff P. M., Davis C., Quintana N. Studies on microperoxisomes. II. A cytochemical method for light and electron microscopy. J Histochem Cytochem. 1972 Dec;20(12):1006–1023. doi: 10.1177/20.12.1006. [DOI] [PubMed] [Google Scholar]
  7. Novikoff A. B., Novikoff P. M. Microperoxisomes. J Histochem Cytochem. 1973 Nov;21(11):963–966. doi: 10.1177/21.11.963. [DOI] [PubMed] [Google Scholar]
  8. Novikoff P. M., Novikoff A. B., Quintana N., Hauw J. J. Golgi apparatus, GERL, and lysosomes of neurons in rat dorsal root ganglia, studied by thick section and thin section cytochemistry. J Cell Biol. 1971 Sep;50(3):859–886. doi: 10.1083/jcb.50.3.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Orci L., Stauffacher W., Rufener C., Lambert A. E., Rouiller C., Renold A. E. Acid phosphatase activity in secretory granules of pancreatic beta cells of normal rats. Diabetes. 1971 Jun;20(6):385–388. doi: 10.2337/diab.20.6.385. [DOI] [PubMed] [Google Scholar]
  10. Palade G. Intracellular aspects of the process of protein synthesis. Science. 1975 Aug 1;189(4200):347–358. doi: 10.1126/science.1096303. [DOI] [PubMed] [Google Scholar]
  11. Shapiro S., Eto S., Fleischer N., Baum S. G. Regulation of in vitro insulin release from a transplantable Syrian hamster insulinoma. Endocrinology. 1975 Aug;97(2):442–447. doi: 10.1210/endo-97-2-442. [DOI] [PubMed] [Google Scholar]
  12. Steiner D. F., Oyer P. E. The biosynthesis of insulin and a probable precursor of insulin by a human islet cell adenoma. Proc Natl Acad Sci U S A. 1967 Feb;57(2):473–480. doi: 10.1073/pnas.57.2.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. VANHEYNINGEN H. E. SECRETION OF PROTEIN BY THE ACINAR CELLS OF THE RAT PANCREAS AS STUDIED BY ELECTRON MICROSCOPIC RADIOAUTOGRAPHY. Anat Rec. 1964 Mar;148:485–497. doi: 10.1002/ar.1091480306. [DOI] [PubMed] [Google Scholar]

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