Abstract
Chick embryo chondrocytes, in primary culture, initially synthesize only Type ii collagen (chain composition, [alpha1(II)13), as judged by two criteria: (i) carbosymethyl-cellulose chromatography of the denatured collagen, and (ii) carbosymethyl-cellulose chromatography of the cyanogen bromide peptides derived from the isolated chains. After a period of growth in 5-bromo-2'-deoxyuridine, however, synthesis of two different types of collagen could be detected after differential salt precipitation of the newly synthesized native collagens from neutral salt solutions at 2.2 M NaCl and subsequently at 0.01 M Na2hPO4. By criteria indicated above, the collagen precipitating at 2.2 M NaCl was identified as Type I collagen (chain composition, [alpha(I)]2alpha2), whereas the collagen subsequently precipitated at 0.01 M Na2HPO4 was found to be comprised entirely of alpha1(I) chains, indicating a chain composition, [alpha](I)]3. We propose to designate the latter type of molecule as the Type I trimer.
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Abbott J., Holtzer H. The loss of phenotypic traits by differentiated cells, V. The effect of 5-bromodeoxyuridine on cloned chondrocytes. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1144–1151. doi: 10.1073/pnas.59.4.1144. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Abbott J., Holtzer H. The loss of phenotypic traits by differentiated cells. 3. The reversible behavior of chondrocytes in primary cultures. J Cell Biol. 1966 Mar;28(3):473–487. doi: 10.1083/jcb.28.3.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bornstein P. The biosynthesis of collagen. Annu Rev Biochem. 1974;43(0):567–603. doi: 10.1146/annurev.bi.43.070174.003031. [DOI] [PubMed] [Google Scholar]
- Chacko S., Abbott J., Holtzer S., Holtzer H. The loss of phenotypic traits by differentiated cells. VI. Behavior of the progeny of a single chondrocyte. J Exp Med. 1969 Aug 1;130(2):417–442. doi: 10.1084/jem.130.2.417. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chung E., Keele E. M., Miller E. J. Isolation and characterization of the cyanogen bromide peptides from the alpha 1(3) chain of human collagen. Biochemistry. 1974 Aug 13;13(17):3459–3464. doi: 10.1021/bi00714a006. [DOI] [PubMed] [Google Scholar]
- Chung E., Miller E. J. Collagen polymorphism: characterization of molecules with the chain composition (alpha 1 (3)03 in human tissues. Science. 1974 Mar;183(130):1200–1201. doi: 10.1126/science.183.4130.1200. [DOI] [PubMed] [Google Scholar]
- Coleman A. W., Coleman J. R., Kankel D., Werner I. The reversible control of animal cell differentiation by the thymidine analog, 5-bromodeoxyuridine. Exp Cell Res. 1970 Feb;59(2):319–328. doi: 10.1016/0014-4827(70)90606-3. [DOI] [PubMed] [Google Scholar]
- Coon H. G. Clonal stability and phenotypic expression of chick cartilage cells in vitro. Proc Natl Acad Sci U S A. 1966 Jan;55(1):66–73. doi: 10.1073/pnas.55.1.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Daniel J. C., Kosher R. A., Lash J. W., Hertz J. The synthesis of matrix components by chondrocytes in vitro in the presence of 5-bromodeoxyuridine. Cell Differ. 1973 Dec;2(5):285–298. doi: 10.1016/0045-6039(73)90033-x. [DOI] [PubMed] [Google Scholar]
- Fessler L. I., Fessler J. H. Protein assembly of procollagen and effects of hydroxylation. J Biol Chem. 1974 Dec 10;249(23):7637–7646. [PubMed] [Google Scholar]
- Hagopian H. K., Lippke J. A., Ingram V. M. Erythropoietic cell cultures from chick embryos. J Cell Biol. 1972 Jul;54(1):98–106. doi: 10.1083/jcb.54.1.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hayflick L. Current theories of biological aging. Fed Proc. 1975 Jan;34(1):9–13. [PubMed] [Google Scholar]
- Lasher R., Cahn R. D. The effects of 5-Bromodeoxyuridine on the differentiation of chondrocytes in vitro. Dev Biol. 1969 May;19(5):415–435. doi: 10.1016/0012-1606(69)90080-3. [DOI] [PubMed] [Google Scholar]
- Martin G. R., Byers P. H., Piez K. A. Procollagen. Adv Enzymol Relat Areas Mol Biol. 1975;42:167–191. doi: 10.1002/9780470122877.ch3. [DOI] [PubMed] [Google Scholar]
- Mayne R., Abbott J., Holtzer H. Requirement for cell proliferation for the effects of 5-bromo-2'-deoxyuridine on cultures of chick chondrocytes. Exp Cell Res. 1973 Mar 15;77(1):255–263. doi: 10.1016/0014-4827(73)90575-2. [DOI] [PubMed] [Google Scholar]
- Mayne R., Sanger J. W., Holtzer H. Inhibition of mucopolysaccharide synthesis by 5-bromodeoxyuridine in cultures of chick amnion cells. Dev Biol. 1971 Aug;25(4):547–567. doi: 10.1016/0012-1606(71)90005-4. [DOI] [PubMed] [Google Scholar]
- Meigel W. N., Müller P. K., Pontz B. F., Sörensen N., Spranger J. A constitutional disorder of connective tissue suggesting a defect in collagen biosynthesis. Klin Wochenschr. 1974 Oct 1;52(19):906–912. doi: 10.1007/BF01468935. [DOI] [PubMed] [Google Scholar]
- Miller E. J. A review of biochemical studies on the genetically distinct collagens of the skeletal system. Clin Orthop Relat Res. 1973 May;(92):260–280. doi: 10.1097/00003086-197305000-00024. [DOI] [PubMed] [Google Scholar]
- Miller E. J., Epstein E. H., Jr, Piez K. A. Identification of three genetically distinct collagens by cyanogen bromide cleavage of insoluble human skin and cartilage collagen. Biochem Biophys Res Commun. 1971 Mar 19;42(6):1024–1029. doi: 10.1016/0006-291x(71)90006-4. [DOI] [PubMed] [Google Scholar]
- Miller E. J., Martin G. R., Piez K. A., Powers M. J. Characterization of chick bone collagen and compositional changes associated with maturation. J Biol Chem. 1967 Dec 10;242(23):5481–5489. [PubMed] [Google Scholar]
- Miller E. J. Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin. Biochemistry. 1972 Dec 19;11(26):4903–4909. doi: 10.1021/bi00776a005. [DOI] [PubMed] [Google Scholar]
- Miller E. J., Woodall D. L., Vail M. S. Biosynthesis of cartilage collagen. Use of pulse labeling to order the cyanogen bromide peptides in the alpha L(II) chain. J Biol Chem. 1973 Mar 10;248(5):1666–1671. [PubMed] [Google Scholar]
- Miura Y., Wilt F. H. The effects of 5-bromodeoxyuridine on yolk sac erythropoiesis in the chick embryo. J Cell Biol. 1971 Mar;48(3):523–532. doi: 10.1083/jcb.48.3.523. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Monson J. M., Borstein P. Identification of a disulfide-linked procollagen as the biosynthetic precursor of chick-bone collagen. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3521–3525. doi: 10.1073/pnas.70.12.3521. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Müller P. K., Meigel W. N., Pontz B. F., Raisch K. Influence of alpha, alpha-dipyridyl on the biosynthesis of collagen in organ cultures. Hoppe Seylers Z Physiol Chem. 1974 Aug;355(8):985–996. doi: 10.1515/bchm2.1974.355.2.985. [DOI] [PubMed] [Google Scholar]
- Nameroff M., Holtzer H. The loss of phenotypic traits by differentiated cells. IV. Changes in polysaccharides produced by dividing chondrocytes. Dev Biol. 1967 Sep;16(3):250–281. doi: 10.1016/0012-1606(67)90026-7. [DOI] [PubMed] [Google Scholar]
- Nimni M., Deshmukh K. Differences in collagen metabolism between normal and osteoarthritic human articular cartilage. Science. 1973 Aug 24;181(4101):751–752. doi: 10.1126/science.181.4101.751. [DOI] [PubMed] [Google Scholar]
- Rutter W. J., Pictet R. L., Morris P. W. Toward molecular mechanisms of developmental processes. Annu Rev Biochem. 1973;42:601–646. doi: 10.1146/annurev.bi.42.070173.003125. [DOI] [PubMed] [Google Scholar]
- Scher W., Preisler H. D., Friend C. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro. 3. Effects of 5-bromo-2'-deoxyuridine, dimethylformamide and dimethylsulfoxide. J Cell Physiol. 1973 Feb;81(1):63–70. doi: 10.1002/jcp.1040810108. [DOI] [PubMed] [Google Scholar]
- Seyer J. M., Brickley D. M., Glimcher M. J. The isolation of two types of collagen from embryonic bovine epiphyseal cartilage. Calcif Tissue Res. 1974;17(1):25–41. doi: 10.1007/BF02547212. [DOI] [PubMed] [Google Scholar]
- Tkocz C., Kühn K. The formation of triple-helical collagen molecules from alpha-1 or alpha-2 polypeptide chains. Eur J Biochem. 1969 Feb;7(4):454–462. doi: 10.1111/j.1432-1033.1969.tb19631.x. [DOI] [PubMed] [Google Scholar]
- Trelstad R. L., Kang A. H., Toole B. P., Gross J. Collagen heterogeneity. High resolution separation of native ( 1(I) 2 2 and ( 1(II) 3 and their component chains. J Biol Chem. 1972 Oct 25;247(20):6469–6473. [PubMed] [Google Scholar]
- Weintraub H., Campbell G. L., Holtzer H. Identification of a developmental program using bromodeoxyuridine. J Mol Biol. 1972 Sep 28;70(2):337–350. doi: 10.1016/0022-2836(72)90543-8. [DOI] [PubMed] [Google Scholar]
- Wilt F. H., Anderson M. The action of 5-bromodeoxyuridine on differentiation. Dev Biol. 1972 Jun;28(2):443–447. doi: 10.1016/0012-1606(72)90026-7. [DOI] [PubMed] [Google Scholar]