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. 1975 Nov;72(11):4602–4606. doi: 10.1073/pnas.72.11.4602

Carcinoembryonic antigen: evidence for multiple antigenic determinants and isoantigens.

R Vrba, E Alpert, K J Isselbacher
PMCID: PMC388771  PMID: 53843

Abstract

Carcinoembryonic antigen (CEA) preparations, from various sources were compared by radioimmunoassay. The preparations studied included four CEA standards (CEA-Roch, CEA-Montreal, CEA-City of Hope, and CEA-British) and CEA from serum and liver metastases of a patient with cancer of the colon who had an extremely high concentration of serum CEA (more than 26,000 ng/ml). The data indicate that the CEA-Roche standard differs significantly from the other three CEA standards tested, and that the serum CEA from the patient was antigenically different from currently available CEA standards as well as from the CEA obtained from the patient's own liver metastases. These antigenic differences were reflected in radioimmunoassay inhibition curves that were different and that were not affected by perchloric acid extraction of CEA. Because of the antigenic variation in the serum CEA, markedly different CEA concentrations (varying by three orders of magnitude) were measurable by two different antisera (Roche and Montreal). All the various CEA standards and samples cochromatographed on columns of Sepharose-6B, despite the large antigenic variation. We postulate that CEA consists of a family of "isoantigens" with multiple antigenic determinants. We identified a serum CEA isoantigen that was different from the currently available standards. Consequently, we believe that results of radioimmunoassays currently used for CEA measurement may not represent absolute concentrations of serum "CEA", but may reflect the binding affinity of different isoantigens to a particular polyvalent CEA antiserum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banjo C., Gold P., Gehrke C. W., Freedman S. O., Krupey J. Preparation and isolation of immunologically active glycopeptides from carcinoembryonic antigen (CEA). Int J Cancer. 1974 Feb 15;13(2):151–163. doi: 10.1002/ijc.2910130202. [DOI] [PubMed] [Google Scholar]
  2. Coligan J. E., Henkart P. A., Todd C. W., Terry W. D. Heterogeneity of the carcinoembryonic antigen. Immunochemistry. 1973 Sep;10(9):591–599. doi: 10.1016/0019-2791(73)90160-2. [DOI] [PubMed] [Google Scholar]
  3. Coligan J. E., Lautenschleger J. T., Egan M. L., Todd C. W. Isolation and characterization of carcinoembryonic antigen. Immunochemistry. 1972 Apr;9(4):377–386. doi: 10.1016/0019-2791(72)90308-4. [DOI] [PubMed] [Google Scholar]
  4. Coligan J. E., Todd C. W. Structural studies on carcinoembryonic antigen. Periodate oxidation. Biochemistry. 1975 Feb 25;14(4):805–810. doi: 10.1021/bi00675a025. [DOI] [PubMed] [Google Scholar]
  5. Edgington T. S., Astarita R. W., Plow E. F. Association of an isomeric species of carcinoembryonic antigen with neoplasia of the gastrointestinal tract. N Engl J Med. 1975 Jul 17;293(3):103–107. doi: 10.1056/NEJM197507172930301. [DOI] [PubMed] [Google Scholar]
  6. Eveleigh J. W. Proceedings: Heterogeneity of carcinoembryonic antigen. Cancer Res. 1974 Aug;34(8):2122–2124. [PubMed] [Google Scholar]
  7. GOLD P., FREEDMAN S. O. DEMONSTRATION OF TUMOR-SPECIFIC ANTIGENS IN HUMAN COLONIC CARCINOMATA BY IMMUNOLOGICAL TOLERANCE AND ABSORPTION TECHNIQUES. J Exp Med. 1965 Mar 1;121:439–462. doi: 10.1084/jem.121.3.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hammarström S., Engvall E., Johansson B. G., Svensson S., Sundblad G., Goldstein I. J. Nature of the tumor-associated determinant(s) of carcinoembryonic antigen. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1528–1532. doi: 10.1073/pnas.72.4.1528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hansen H. J., Snyder J. J., Miller E., Vandevoorde J. P., Miller O. N., Hines L. R., Burns J. J. Carcinoembryonic antigen (CEA) assay. A laboratory adjunct in the diagnosis and management of cancer. Hum Pathol. 1974 Mar;5(2):139–147. doi: 10.1016/s0046-8177(74)80061-4. [DOI] [PubMed] [Google Scholar]
  10. Krupey J., Wilson T., Freedman S. O., Gold P. The preparation of purified carcinoembryonic antigen of the human digestive system from large quantities of tumor tissue. Immunochemistry. 1972 Jun;9(6):617–622. doi: 10.1016/0019-2791(72)90247-9. [DOI] [PubMed] [Google Scholar]
  11. Plow E. F., Edgington T. S. Isolation and characterization of a homogeneous isomeric species of carcinoembryonic antigen: cea-s. Int J Cancer. 1975 May 15;15(5):748–761. doi: 10.1002/ijc.2910150506. [DOI] [PubMed] [Google Scholar]
  12. Rule A. H., Goleski-Reilly C. Carcinoembryonic antigen (CEA): separation of CEA-reacting molecules from tumor, fetal gut, meconium and normal colon. Immunol Commun. 1973;2(2):213–226. doi: 10.3109/08820137309022793. [DOI] [PubMed] [Google Scholar]
  13. Sorokin J. J., Kupchik H. Z., Zamcheck N., Dhar P. A clinical comparison of two radioimmunoassays for carcinoembryonic antigen (CEA). Immunol Commun. 1972;1(1):11–24. doi: 10.3109/08820137209022889. [DOI] [PubMed] [Google Scholar]
  14. Thomson D. M., Krupey J., Freedman S. O., Gold P. The radioimmunoassay of circulating carcinoembryonic antigen of the human digestive system. Proc Natl Acad Sci U S A. 1969 Sep;64(1):161–167. doi: 10.1073/pnas.64.1.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Turner M. D. Carcinoembryonic antigen. JAMA. 1975 Feb 17;231(7):756–758. [PubMed] [Google Scholar]
  16. Vrba R., Cannon W. Molecular weights and metabolism of rat brain proteins. Biochem J. 1970 Feb;116(4):745–753. doi: 10.1042/bj1160745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Westwood J. H., Bessell E. M., Bukhari M. A., Thomas P., Walker J. M. Studies on the structure of the carcinoembryonic antigen. I. some deductions on the basis of chemical degradations. Immunochemistry. 1974 Dec;11(12):811–818. doi: 10.1016/0019-2791(74)90302-4. [DOI] [PubMed] [Google Scholar]
  18. von Kleist S., Chavanel G., Burtin P. Identification of an antigen from normal human tissue that crossreacts with the carcinoembryonic antigen. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2492–2494. doi: 10.1073/pnas.69.9.2492. [DOI] [PMC free article] [PubMed] [Google Scholar]

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