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. 1975 Dec;72(12):4777–4779. doi: 10.1073/pnas.72.12.4777

Translation of messenger ribonucleic acid from isolated pancreatic islets and human insulinomas.

C C Yip, C L Hew, H Hsu
PMCID: PMC388814  PMID: 174093

Abstract

RNA preparations from isolated rat pancreatic islets and from human insulinomas were injected into oocytes of Xenopus laevis which were then incubated with [3H]leucine. Acid-ethanol extracts of the oocytes were immunoprecipitated with anti-insulin serum using the double antibody technique. Sodium dodecyl sulfate disc gel electrophoresis of the immunoprecipitates showed the presence of an insulin-displaceable immunoreactive material with a molecular weight of about 18,000 in extracts from oocytes injected with RNA of 9-11 S. This immunoreactive product was not detected in extracts from oocytes injected with buffer or 4-8S RNA or RNA heavier than 11 S. These observations suggest the involvement of a precursor larger than proinsulin in the biosynthesis of insulin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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