Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Dec;72(12):4780–4784. doi: 10.1073/pnas.72.12.4780

Content of elongation factor Tu in Escherichia coli.

A V Furano
PMCID: PMC388815  PMID: 1108000

Abstract

The content of elongation factor Tu in E. coli B has been determined both by radioimmune assay and by GDP binding. The two assays gave comparable results: cells growing at 2 doublings per hour contained about 8 molecules of Tu per ribosome, whereas those growing at 0.22 doublings per hour contained about 14 molecules per ribosome. These levels resemble those reported for tRNA, in contrast with the 1:1 ratio of factor to ribosomes reported for elongation factors Ts and G.

Full text

PDF
4780

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allende J. E., Seeds N. W., Conway T. W., Weissbach H. Guanosine triphosphate interaction with an amino acid polymerization factor from E. coli. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1566–1573. doi: 10.1073/pnas.58.4.1566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson E. H. Growth Requirements of Virus-Resistant Mutants of Escherichia Coli Strain "B". Proc Natl Acad Sci U S A. 1946 May;32(5):120–128. doi: 10.1073/pnas.32.5.120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Arai K. I., Kawakita M., Kaziro Y. Studies on polypeptide elongation factors from Escherichia coli. II. Purification of factors Tu-guanosine diphosphate, Ts, and Tu-Ts, and crystallization of Tu-guanosine diphosphate and Tu-Ts. J Biol Chem. 1972 Nov 10;247(21):7029–7037. [PubMed] [Google Scholar]
  4. Baltimore D. Purification and properties of poliovirus double-stranded ribonucleic acid. J Mol Biol. 1966 Jul;18(3):421–428. doi: 10.1016/s0022-2836(66)80034-7. [DOI] [PubMed] [Google Scholar]
  5. Blumenthal T., Landers T. A., Weber K. Bacteriophage Q replicase contains the protein biosynthesis elongation factors EF Tu and EF Ts. Proc Natl Acad Sci U S A. 1972 May;69(5):1313–1317. doi: 10.1073/pnas.69.5.1313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dennis P. P., Bremer H. Macromolecular composition during steady-state growth of Escherichia coli B-r. J Bacteriol. 1974 Jul;119(1):270–281. doi: 10.1128/jb.119.1.270-281.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Furano A. V. A very rapid method for washing large numbers of precipitates of proteins and nucleic acids. Anal Biochem. 1971 Oct;43(2):639–640. doi: 10.1016/0003-2697(71)90300-9. [DOI] [PubMed] [Google Scholar]
  8. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gordon J. Regulation of the in vivo synthesis of the polypeptide chain elongation factors in Escherichia coli. Biochemistry. 1970 Feb 17;9(4):912–917. doi: 10.1021/bi00806a028. [DOI] [PubMed] [Google Scholar]
  10. Gordon J., Weissbach H. Immunochemical distinction between the Escherichia coli polypeptide chain elongation factors Tu and Ts. Biochemistry. 1970 Oct 13;9(21):4233–4236. doi: 10.1021/bi00823a028. [DOI] [PubMed] [Google Scholar]
  11. Haselkorn R., Rothman-Denes L. B. Protein synthesis. Annu Rev Biochem. 1973;42:397–438. doi: 10.1146/annurev.bi.42.070173.002145. [DOI] [PubMed] [Google Scholar]
  12. Jaskunas S. R., Lindahl L., Nomura M. Identification of two copies of the gene for the elongation factor EF-Tu in E. coli. Nature. 1975 Oct 9;257(5526):458–462. doi: 10.1038/257458a0. [DOI] [PubMed] [Google Scholar]
  13. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  14. LEVY H. B., SOBER H. A. A simple chromatographic method for preparation of gamma globulin. Proc Soc Exp Biol Med. 1960 Jan;103:250–252. doi: 10.3181/00379727-103-25476. [DOI] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Miller D. L., Weissbach H. Studies on the purification and properties of factor Tu from E. coli. Arch Biochem Biophys. 1970 Nov;141(1):26–37. doi: 10.1016/0003-9861(70)90102-5. [DOI] [PubMed] [Google Scholar]
  17. Neville D. M., Jr Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system. J Biol Chem. 1971 Oct 25;246(20):6328–6334. [PubMed] [Google Scholar]
  18. Rosset R., Julien J., Monier R. Ribonucleic acid composition of bacteria as a function of growth rate. J Mol Biol. 1966 Jul;18(2):308–320. doi: 10.1016/s0022-2836(66)80248-6. [DOI] [PubMed] [Google Scholar]
  19. Skjold A. C., Juarez H., Hedgcoth C. Relationships among deoxyribonucleic acid, ribonucleic acid, and specific transfer ribonucleic acids in Escherichia coli 15T - at various growth rates. J Bacteriol. 1973 Jul;115(1):177–187. doi: 10.1128/jb.115.1.177-187.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Travers A. Control of ribosomal RNA synthesis in vitro. Nature. 1973 Jul 6;244(5410):15–18. doi: 10.1038/244015a0. [DOI] [PubMed] [Google Scholar]
  21. Weissbach H., Redfield B., Brot N. Further studies on the role of factors Ts and Tu in protein synthesis. Arch Biochem Biophys. 1971 May;144(1):224–229. doi: 10.1016/0003-9861(71)90472-3. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES