Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Dec;72(12):4844–4848. doi: 10.1073/pnas.72.12.4844

Regulation of synthesis of glutamine synthetase by adenylylated glutamine synthetase.

F Foor, K A Janssen, B Magasanik
PMCID: PMC388828  PMID: 1744

Abstract

We have examined three mutants of Klebsiella aerogenes whose genetic lesions (glnB, glnD, and glnE) are in loci unlinked to the structural gene for glutamine sythetase (glnA) and in which the control of both the level and state of adenylylation of glutamine synthetase is altered. Each mutation alters a different component of the adenylylation system of glutamine synthetase [L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2]. Inability of the cell to deadenylylate glutamine synthetase (glnB and glnD) greatly decreases its production, while inability to adenylylate glutamine sythetase (glnE) results in its constitutively high production. These results together with our previous results indicate that adenylylated glutamine synthetase inhibits the transcription of glnA.

Full text

PDF
4844

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson W. B., Hennig S. B., Ginsburg A., Stadtman E. R. Association of ATP: glutamine synthetase adenylyltransferase activity with the P1 component of the glutamine synthetase deadenylylation system. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1417–1424. doi: 10.1073/pnas.67.3.1417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Deleo A. B., Magasanik B. Identification of the structural gene for glutamine synthetase in Klebsiella aerogenes. J Bacteriol. 1975 Jan;121(1):313–319. doi: 10.1128/jb.121.1.313-319.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Prival M. J., Brenchley J. E., Magasanik B. Glutamine synthetase and the regulation of histidase formation in Klebsiella aerogenes. J Biol Chem. 1973 Jun 25;248(12):4334–4344. [PubMed] [Google Scholar]
  4. Shapiro B. M. The glutamine synthetase deadenylylating enzyme system from Escherichia coli. Resolution into two components, specific nucleotide stimulation, and cofactor requirements. Biochemistry. 1969 Feb;8(2):659–670. doi: 10.1021/bi00830a030. [DOI] [PubMed] [Google Scholar]
  5. Stadtman E. R., Ginsburg A., Ciardi J. E., Yeh J., Hennig S. B., Shapiro B. M. Multiple molecular forms of glutamine synthetase produced by enzyme catalyzed adenylation and deadenylylation reactions. Adv Enzyme Regul. 1970;8:99–118. doi: 10.1016/0065-2571(70)90011-7. [DOI] [PubMed] [Google Scholar]
  6. Streicher S. L., Bender R. A., Magasanik B. Genetic control of glutamine synthetase in Klebiella aerogenes. J Bacteriol. 1975 Jan;121(1):320–331. doi: 10.1128/jb.121.1.320-331.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Tyler B., Deleo A. B., Magasanik B. Activation of transcription of hut DNA by glutamine synthetase. Proc Natl Acad Sci U S A. 1974 Jan;71(1):225–229. doi: 10.1073/pnas.71.1.225. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES